The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Xanthogenate nucleic acid isolation from cultured and environmental cyanobacteria
Abstract
The isolation of high‐quality nucleic acids from cyanobacterial strains, in particular environmental isolates, has proven far from trivial. We present novel techniques for the extraction of high molecular weight DNA and RNA from a range of cultured and environmental cyanobacteria, including stains belonging to the genera Microcystis, Lyngbya, Pseudanabaena, Aphanizomenon, Nodularia, Anabaena, and Nostoc, based on the use of the nontoxic polysaccharide solubilizing compound xanthogenate. These methods are rapid, require no enzymatic or mechanical cell disruption, and have been used to isolate both DNA and RNA free of enzyme inhibitors or nucleases. In addition, these procedures have proven critical in the molecular analysis of bloom‐forming and other environmental cyanobacterial isolates. Finally, these techniques are of general microbiological utility for a diverse range of noncyanobacterial microorganisms, including Gram‐positive and Gram‐negative bacteria and the Archea.
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Cites
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Lehmann, M., Vamvaka, E., Torrado, A., Jahns, P., Dann, M., Rosenhammer, L., Aziba, A., Leister, D., & Rühle, T. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.699424 · Cited by 17
Methods for extracting ’omes from microbialites
Gómez-Acata, E. S., Centeno, C. M., & Falcón, L. I. (2019) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2019.02.014 · Cited by 15
Metadata
- Authors
- Tillett, D., & Neilan, B. A.
- Venue
- Journal of Phycology
- Publisher
- Wiley
- Published
- 2000-02-09
- Volume
- 36
- Issue
- 1
- Pages
- 251-258
- ISSN
- 0022-3646 (ISSN-L)
- 1529-8817
- OpenAlex
- W2143705407
- Cited by
- 355
- References
- 32
- FWCI
- 8.30
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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