The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Use of degenerate oligonucleotides for amplification of the nifH gene from the marine cyanobacterium Trichodesmium thiebautii
Open accessAbstract
Trichodesmium spp. are marine filamentous, nonheterocystous, nitrogen-fixing cyanobacteria which are an important component of marine ecosystems. This organism has never been maintained in axenic culture, and there has remained some doubt as to the identity of the organism responsible for nitrogen fixation in Trichodesmium aggregates. By using degenerate oligonucleotide primers, it has been possible to amplify, clone, and sequence a segment of the nifH gene from a natural assemblage of Trichodesmium thiebautii. Examination of the DNA and presumed amino acid sequence shows that the gene is most closely related to that of Anabaena spp. and therefore is most likely a cyanobacterial nifH gene. The use of degenerate oligonucleotides, in concert with the polymerase chain reaction, can be a powerful tool for the cloning and sequencing of a variety of genes from microorganisms in the environment.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Cited by
High rates of N2 fixation by unicellular diazotrophs in the oligotrophic Pacific Ocean
Montoya, J. P., Holl, C. M., Zehr, J. P., Hansen, A., Villareal, T. A., & Capone, D. G. (2004) · Nature · doi:10.1038/nature02824 · Cited by 559
Self-citationFan, K., Weisenhorn, P., Gilbert, J. A., Shi, Y., Bai, Y., & Chu, H. (2018) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2018.03.017 · Cited by 398
Zehr, J. P., Mellon, M. T., & Zani, S. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.9.3444-3450.1998 · Cited by 363
Self-citationXanthogenate nucleic acid isolation from cultured and environmental cyanobacteria
Tillett, D., & Neilan, B. A. (2000) · Journal of Phycology · doi:10.1046/j.1529-8817.2000.99079.x · Cited by 355
Farías, M. E., Rascovan, N., Toneatti, D. M., Albarracín, V. H., Flores, M. R., Poiré, D. G., Collavino, M. M., Aguilar, O. M., Vazquez, M. P., & Polerecky, L. (2013) · PLoS ONE · doi:10.1371/journal.pone.0053497 · Cited by 133
Metadata
- Authors
- Zehr, J. P., & McReynolds, L. A.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1989-10
- Volume
- 55
- Issue
- 10
- Pages
- 2522-2526
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1530752960
- Cited by
- 497
- References
- 2
- FWCI
- 8.89
- Type
- journal article
- Language
- en
- Open access
- green
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- Zehr, J. P., & McReynolds, L. A. (1989). Use of degenerate oligonucleotides for amplification of the nifH gene from the marine cyanobacterium Trichodesmium thiebautii. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.55.10.2522-2526.1989
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