CoChat
Sign Up
Cited by 121Polysaccharides Composition and ApplicationsProbiotics and Fermented FoodsProteins in Food Systems

The DOI matches its Crossref record; 33 of the 39 references it cites were found in a registry or an index.

Comparative study of different methodologies to determine the exopolysaccharide produced by kefir grains in milk and whey

Open access
Rimada, Pablo Sebasti�n, Abraham, Anal�a Graciela·2003·Le Lait
DOI 10.1051/lait:2002051
Open this paper in CoChat — free accountOpen PDF

Abstract

Quantitative isolation of lactic acid bacteria exopolysaccharides (EPS) from dairy products is an important step to determine small amounts of EPS (between 25 and 890 mg•L -1 ) in a medium that contains 30 to 50 g•L -1 of lactose.Kefir grains CIDCA AGK1 are able to produce an exopolysaccharide commonly known as kefiran when grown in milk and deproteinised whey.Different methodologies were compared for kefiran isolation from milk and whey fermented for 96 h at 20 °C with 100 g•L -1 of kefir grains.Methods that included one or two steps of ethanol precipitation, one step of ethanol precipitation followed by dialysis, direct dialysis with membranes of different cut-off (1 000, 6 000, 12 000), and a TCA precipitation step were evaluated.The effect of a heat treatment of the milk on EPS recovery was also studied.The highest recovery of EPS was obtained when samples were heated as a first step of isolation.Methods that contained two steps of ethanol precipitation, one step of ethanol precipitation followed by dialysis or direct dialysis (molecular weight cut-off lower than 6 000 to 8 000) gave the highest values of polysaccharide concentration (218 mg•L -1 of fermented milk and 247 mg•L -1 of fermented whey).One step of ethanol precipitation did not completely eliminate residual lactose.EPS was partially lost when dialysis was carried out with membranes of cut-off of 12 000.About 50% of EPS was lost when the method included a step of TCA precipitation.We conclude that polysaccharide quantified in milk and deproteinised whey fermented with kefir grains strongly depends on the isolation methodology used. Exopolysaccharide / EPS isolation / fermented milk / fermented whey / kefirRésumé -Étude comparative de différentes méthodologies pour déterminer la quantité d'exopolysaccharide produit par les grains de kefir dans le lait et le lactosérum.L'isolement quantitatif des exopolysaccharides (EPS) de bactéries lactiques à partir des produits laitiers est une étape importante pour déterminer de petites quantités d'EPS (entre 25 et 890 mg•L -1 ) dans un milieu contenant entre 30 et 50 g•L -1 de lactose.Quand les grains de kefir AGK1 croissent dans le lait et dans le lactosérum deprotéiné, ils sont capables de produire un EPS généralement connu comme kefiran.Dans ce travail, nous avons comparé différentes méthodologies pour isoler le kefiran dans du lait et du lactosérum fermentés.Pour réaliser ces essais le lait et le lactoserum ont été fermentés pendant 96 h 20 °C avec 100 g•L -1 de grains de kefir.Les différentes méthodologies pour déterminer l'EPS sont: la précipitation à l'ethanol, la précipitation à l'ethanol suivie d'une dialyse, une dialyse directe

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1051/lait:2002051

Cites

33 of 33 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Research works printed but not linked: 1

  1. Zweig G., Sherma J., Handbook Series in Chromatography, Section A: General Data and Principles Volume II, CRC Press Inc., Boca Raton, USA, 1978, pp. 124-130.

Cited by

Sorted by citations

See all 121

Related

See all

Metadata

Authors
Rimada, P. S., & Abraham, A. G.
Venue
Le Lait
Publisher
EDP Sciences
Published
2003-01
Volume
83
Issue
1
Pages
79-87
ISSN
  • 0023-7302 (ISSN-L)
  • 1297-9694
DOI
10.1051/lait:2002051
OpenAlex
W2120005006
Cited by
121
References
39
Citations by year
2026: 3 · 2025: 9 · 2024: 6 · 2023: 5 · 2022: 12 · 2021: 6 · 2020: 10 · 2019: 7 · 2018: 9 · 2017: 10 · 2016: 5 · 2015: 8 · 2013: 5 · 2012: 5
FWCI
2.76
Type
journal article
Language
en
Open access
Locations
  • Le Lait (publishedVersion)
  • HAL (submittedVersion, other-oa)
  • CONICET Digital (CONICET) (submittedVersion, cc-by-nc-sa)
Funders
  • Consejo Nacional de Investigaciones Científicas y Técnicas
  • Universidad Nacional de La Plata
  • Agencia Nacional de Promoción Científica y Tecnológica (CONICET)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
Keywords
kefiran, kefir grains, lactic acid bacteria, fermented milk, ethanol precipitation, dialysis, TCA precipitation, heat treatment (food and biological processing), lactose removal, polysaccharide recovery, molecular weight cut-off membranes, dairy products, kefir, fermentation medium composition

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review