Cited by 61Parasitic Infections and DiagnosticsHelminth infection and controlViral gastroenteritis research and epidemiology

Molecular identification of Giardia and Cryptosporidium from dogs and cats

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Sotiriadou, Isaia, Pantchev, Nikola, Gassmann, Doreen, Karanis, Panagiotis·2013·Parasite

Abstract

The aim of the present study was to diagnose the presence of Giardia cysts and Cryptosporidium oocysts in household animals using nested polymerase chain reaction (PCR) and sequence analysis. One hundred faecal samples obtained from 81 dogs and 19 cats were investigated. The Cryptosporidium genotypes were determined by sequencing a fragment of the small subunit (SSU) rRNA gene, while the Giardia Assemblages were determined through analysis of the glutamate dehydrogenase (GDH) locus. Isolates from five dogs and two cats were positive by PCR for the presence of Giardia, and their sequences matched the zoonotic Assemblage A of Giardia. Cryptosporidium spp. isolated from one dog and one cat were both found to be C. parvum. One dog isolate harboured a mixed infection of C. parvum and Giardia Assemblage A. These findings support the growing evidence that household animals are potential reservoirs of the zoonotic pathogens Giardia spp. and Cryptosporidium spp. for infections in humans.

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Authors
Sotiriadou, I., Pantchev, N., Gassmann, D., & Karanis, P.
Affiliations
  • University of Cologne (DE)
  • IDEXX Laboratories (Germany) (DE)
Corresponding author
Sotiriadou, Isaia
Venue
Parasite
Publisher
EDP Sciences
Published
2013
Volume
20
Pages
8
ISSN
  • 1252-607X (ISSN-L)
  • 1776-1042
OpenAlex
W2063780704
Cited by
61
References
53
Citations by year
2026: 1 · 2025: 4 · 2024: 4 · 2023: 7 · 2022: 3 · 2021: 7 · 2020: 2 · 2019: 1 · 2018: 4 · 2017: 8 · 2016: 4 · 2015: 9 · 2014: 4 · 2013: 3
FWCI
5.66
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Parasite (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Zenodo (CERN European Organization for Nuclear Research) (submittedVersion, public-domain)
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Parasitic Infections and Diagnostics
  • Health Sciences › Veterinary › Small Animals › Helminth infection and control
  • Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
Keywords
Giardia, Cryptosporidium, Cryptosporidium parvum, dogs, cats, zoonotic parasites, Giardia assemblage A, co-infection, small subunit rRNA gene, fecal samples, molecular diagnosis
MeSH terms
Animals, Base Sequence, Cat Diseases, Cats, Cryptosporidiosis, Cryptosporidium, Dog Diseases, Dogs, Feces, Giardia, Giardiasis, Glutamate Dehydrogenase, Molecular Sequence Data, Phylogeny, DNA, Protozoan, Polymerase Chain Reaction, Sequence Alignment, Oocysts

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