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Cited by 526Viral gastroenteritis research and epidemiologyGlycosylation and Glycoproteins ResearchBlood groups and transfusion

Norwalk virus binds to histo-blood group antigens present on gastroduodenal epithelial cells of secretor individuals

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Marionneau, Severine, Ruvoën, Nathalie, Le Moullac–Vaidye, Beatrice, Clement, Monique, Cailleau–Thomas, Anne, Ruiz–Palacois, Guillermo, Huang, Pengwei, Jiang, Xi +1 more·2002·Gastroenterology
DOI 10.1053/gast.2002.33661PMC PMC7172544
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Abstract

Background & aims Norwalk Virus (NV) is a member of the Caliciviridae family, which causes acute epidemic gastroenteritis in humans of all ages and its cellular receptors have not yet been characterized. Another calicivirus, Rabbit Hemorrhagic Disease Virus, attaches to H type 2 histo-blood group oligosaccharide present on rabbit epithelial cells. Our aim was to test if, by analogy, recombinant NV-like particles (rNV VLPs) use carbohydrates present on human gastroduodenal epithelial cells as ligands. Methods Attachment of rNV VLPs was tested on tissue sections of the gastroduodenal junction and on saliva from individuals of known ABO, Lewis, and secretor phenotypes. It was also tested on human Caco-2 cells and on animal cell lines transfected with glycosyltransferases complementary DNA (cDNA). Competition experiments were performed with synthetic oligosaccharides and anticarbohydrate antibodies. Internalization was monitored by confocal microscopy. Results Attachment of rNV VLPs to surface epithelial cells of the gastroduodenal junction as well as to saliva was detected, yet only from secretor donors. It was abolished by alpha1,2fucosidase treatment, and by competition with the H types 1 and 3 trisaccharides or with anti-H type 1 and anti-H types (3/4) antibodies. Transfection of CHO and TS/A cells with an alpha1,2fucosyltransferase cDNA allowed attachment of VLPs. These transfectants as well as differentiated Caco-2 cells expressing H type 1 structures internalized the bound particles. Conclusions rNV VLPs use H type 1 and/or H types (3/4) as ligands on gastroduodenal epithelial cells of secretor individuals.

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  • RHDV and human noroviruses bind histo-blood group antigens - complex carbohydrates linked to glycoproteins or glycolipids on the surface of erythrocytes and mucosal epithelial cells.

    “while RHDV and human noroviruses bind histo-blood group antigens – complex carbohydrates linked to glycoproteins or glycolipids on the surface of erythrocytes and mucosal epithelial cells [40–42].”

    From Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures (2017) · cites this paper as [40]

    The Abstract describes RHDV binding an H-type histo-blood group antigen and identifies these antigens as carbohydrates on glycoproteins or glycolipids. The Discussion reports that Norwalk virus particles bind H-type antigens on cells, but explicitly says whether other human norovirus strains do so remains unknown. The claim therefore extends the finding beyond the strain tested; our copy provides no page numbers for these passages. (LLM, conf=0.87)

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Authors
Marionneau, S., Ruvoën, N., Le Moullac–Vaidye, B., Clement, M., Cailleau–Thomas, A., Ruiz–Palacois, G., Huang, P., Jiang, X., & Le Pendu, J.
Affiliations
  • Inserm (FR)
  • Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (MX)
  • Eastern Virginia Medical School (US)
ORCID
Venue
Gastroenterology
Publisher
Elsevier BV
Published
2002-06
Volume
122
Issue
7
Pages
1967-1977
ISSN
0016-5085 (ISSN-L)
DOI
10.1053/gast.2002.33661
PMC
PMC7172544
OpenAlex
W1998368194
Cited by
526
References
1
Citations by year
2026: 16 · 2025: 19 · 2024: 13 · 2023: 20 · 2022: 16 · 2021: 28 · 2020: 21 · 2019: 27 · 2018: 22 · 2017: 21 · 2016: 35 · 2015: 26 · 2014: 32 · 2013: 25 · 2012: 21
FWCI
11.79
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Gastroenterology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Institut National de la Santé et de la Recherche Médicale
Topics
  • Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
  • Health Sciences › Medicine › Hematology › Blood groups and transfusion
Keywords
Norwalk virus, calicivirus, histo-blood group antigens, H antigen, secretor status, viral receptor, viral attachment, viral internalization, virus-like particles, alpha-1,2-fucosyltransferase, ABO blood group system, Lewis blood group system, Caco-2 cells, viral gastroenteritis
MeSH terms
ABO Blood-Group System, Animals, Blood Group Antigens, Carbohydrates, Duodenum, Epithelial Cells, Cricetinae, Humans, Isoantigens, Lewis Blood Group Antigens, Ligands, Norwalk virus, Phenotype, Saliva, Stomach, Virion, CHO Cells, Caco-2 Cells, Rats

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