Flow–Cytometric Separation and Enrichment of Hepatic Progenitor Cells in the Developing Mouse Liver
Abstract
Stem cells responsible for tissue maintenance and repair are found in a number of organs. However, hepatic stem cells assumed to play a key role in liver development and regeneration remain to be well characterized. To address this issue, we set up a culture system in which primitive hepatic progenitor cells formed colonies. By combining this culture system with fluorescence-activated cell sorting (FACS), cells forming colonies containing distinct hepatocytes and cholangiocytes were identified in the fetal mouse liver. These cells express both CD49f and CD29 (alpha6 and beta1 integrin subunits), but do not mark for hematopoietic antigens such as CD45, TER119, and c-Kit. When transplanted into the spleen, these cells migrated to the recipient liver and differentiated into liver parenchymal cells. Our data demonstrate that hepatic progenitor cells are enriched by FACS and suggest approaches to supplanting organ allografting and improving artificial-organ hepatic support.
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Isolating c-Met+ c-Kit- CD45- Ter119- cells achieves a much higher enrichment of hepatoblasts.
“Isolating c-Met + c-Kit - CD45 -Ter119 -(also known as Met, Kit, Ptprc and Ly76, respectively -Mouse Genome Informatics) cells achieved a much higher enrichment of hepatoblasts, and thus this method could facilitate the identification of a discrete set of transcription factors that are activated in this specific cell population.”
From Tbx3 controls the fate of hepatic progenitor cells in liver development by suppressing <i>p19ARF</i> expression (2008) · cites this paper as Suzuki et al., 2000
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Jiang, Y., Feng, D., Ma, X., Fan, S., Gao, Y., Fu, K., Wang, Y., Sun, J., Yao, X., Liu, C., et al. (2019) · Hepatology · doi:10.1002/hep.30131 · Cited by 103 · see citing works
Tsuchiya, A., Heike, T., Fujino, H., Shiota, M., Umeda, K., Yoshimoto, M., Matsuda, Y., Ichida, T., Aoyagi, Y., & Nakahata, T. (2005) · Gastroenterology · doi:10.1053/j.gastro.2005.03.030 · Cited by 43 · see citing works
- Authors
- Suzuki, A., Zheng, Y.-W., Kondo, R., Kusakabe, M., Takada, Y., Fukao, K., Nakauchi, H., & Taniguchi, H.
- Affiliations
- University of Tsukuba (JP)
- Corresponding author
- Taniguchi, Hideki
- ORCID
- Venue
- Hepatology
- Published
- 2000-12
- Volume
- 32
- Issue
- 6
- Pages
- 1230-1239
- ISSN
- 0270-9139 (ISSN-L)
- OpenAlex
- W1975655671
- Cited by
- 294
- References
- 0
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 5 · 2022: 1 · 2021: 1 · 2020: 10 · 2019: 7 · 2018: 10 · 2017: 8 · 2016: 8 · 2015: 12 · 2014: 11 · 2013: 16 · 2012: 11
- FWCI
- 12.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Hepatology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Grant-in-aid for Developmental Scientific Research, The Ministry of Education, Science and Culture
- “Research for the Future” Program
- Japan Society for the Promotion of Science (JSPS) (JSPS-RFTF96I00202)
- CREST of Japan Science and Technology Corporation (JST)
- Uehara Memorial Foundation, the Kanehara Ichiro Memorial Foundation, the Cell Science Research Foundation
- Organized Research Combination System from the Science and Technology Agency of Japan
- Topics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Keywords
- hepatic progenitor cells, liver development, cell enrichment, colony-forming cells, hepatocytes, cholangiocytes, CD49f, beta1 integrin, integrins, cell transplantation, liver regeneration, artificial liver support
- MeSH terms
- Animals, Cell Differentiation, Cell Division, Cell Movement, Cell Separation, Flow Cytometry, Liver, Mice, Inbred BALB C, Spleen, Stem Cells, Antigens, CD, Integrin beta1, Hepatocytes, Stem Cell Transplantation, Integrin alpha6, Mice
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