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Cited by 356Liver physiology and pathologyMesenchymal stem cell researchHematopoietic Stem Cell Transplantation

Clonal identification and characterization of self-renewing pluripotent stem cells in the developing liver

Open access
Suzuki, Atsushi, Zheng, Yun-wen, Kaneko, Shin, Onodera, Masafumi, Fukao, Katashi, Nakauchi, Hiromitsu, Taniguchi, Hideki·2002·The Journal of Cell Biology
DOI 10.1083/jcb.200108066PMC PMC2173576
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Abstract

Using flow cytometry and single cell–based assays, we prospectively identified hepatic stem cells with multilineage differentiation potential and self-renewing capability. These cells could be clonally propagated in culture where they continuously produced hepatocytes and cholangiocytes as descendants while maintaining primitive stem cells. When cells that expanded in vitro were transplanted into recipient animals, they morphologically and functionally differentiated into hepatocytes and cholangiocytes with reconstitution of hepatocyte and bile duct structures. Furthermore, these cells differentiated into pancreatic ductal and acinar cells or intestinal epithelial cells when transplanted into pancreas or duodenal wall. These data indicate that self-renewing pluripotent stem cells persist in the developing mouse liver and that such cells can be induced to become cells of other organs of endodermal origin under appropriate microenvironment. Manipulation of hepatic stem cells may provide new insight into therapies for diseases of the digestive system.

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Crossref · 10.1083/jcb.200108066

Cites

42 of 42 references verified0 citation errors · 0 not checkable

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Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Isolating c-Met+ c-Kit- CD45- Ter119- cells achieves a much higher enrichment of hepatoblasts.

    “Isolating c-Met + c-Kit - CD45 -Ter119 -(also known as Met, Kit, Ptprc and Ly76, respectively -Mouse Genome Informatics) cells achieved a much higher enrichment of hepatoblasts, and thus this method could facilitate the identification of a discrete set of transcription factors that are activated in this specific cell population.”

    From Tbx3 controls the fate of hepatic progenitor cells in liver development by suppressing <i>p19ARF</i> expression (2008) · cites this paper as Suzuki et al., 2002

    The Results section, “Flow cytometric fractionation of fetal mouse liver cells” (page unavailable), reports 560-fold enrichment of hepatic colony-forming cells. But that enrichment required sorting for CD49f+/low as well as the four markers named in the claim; the paper does not establish the same enrichment for the four-marker selection alone. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • c-Met+ cells display self-renewing cell divisions and differentiation into both hepatocytes and cholangiocytes in response to Hgf stimulus.

    “In particular, cells marked by the hepatocyte growth factor (Hgf) receptor c-Met displayed distinctive activities in response to Hgf stimulus, including self-renewing cell divisions and differentiation into both hepatocytes and cholangiocytes (Suzuki et al., 2002;Suzuki et al., 2003a).”

    From Tbx3 controls the fate of hepatic progenitor cells in liver development by suppressing <i>p19ARF</i> expression (2008) · cites this paper as Suzuki et al., 2002

    The Results (Flow cytometric fractionation; Clonal expansion and self-renewal capability) identify self-renewing, multilineage H-CFU-C within the c-Met+ cell subpopulation and state that HGF is required for their proliferation. The Discussion (Self-renewal of H-CFU-C in vitro and in vivo) reports divisions yielding both hepatocytes and cholangiocytes, but the cited passages do not establish that HGF stimulus drives differentiation into both lineages. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

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Cites

Flagged first, then by citations

1 of the 43 references couldn’t be matched to a known paper.

Research works printed but not linked: 3

  1. Fausto, N. 1994. Liver stem cells. The Liver. 3rd ed. I.M. Arias, J.L. Boyer, N. Fausto, W.B. Jakoby, D.A. Schachter, and D.A. Shafritz, editors. Ed. 3. Raven Press, New York. 1501–1518.
  2. Fausto, N., and E.M. Webber. 1994. Liver regeneration. The Liver. 3rd ed. I.M. Arias, J.L. Boyer, N. Fausto, W.B. Jakoby, D.A. Schachter, and D.A. Shafritz, editors. Raven Press, New York. 1059–1084.
  3. Metcalf, D., and M.A.S. Moore. 1971. Hematopoietic cells. Frontiers of Biology. A. Neuberger and E.L. Tatum, editors. Vol. 25. North-Holland, Amsterdam.

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Related

Authors
Suzuki, A., Zheng, Y.-W., Kaneko, S., Onodera, M., Fukao, K., Nakauchi, H., & Taniguchi, H.
Affiliations
University of Tsukuba (JP)
ORCID
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
2002-01-07
Volume
156
Issue
1
Pages
173-184
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.200108066
PMC
PMC2173576
OpenAlex
W2121054813
Cited by
356
References
43
Citations by year
2025: 2 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 1 · 2020: 7 · 2019: 8 · 2018: 10 · 2017: 9 · 2016: 5 · 2015: 4 · 2014: 14 · 2013: 19 · 2012: 15
FWCI
20.45
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
Keywords
hepatic stem cells, pluripotent stem cells, self-renewal, clonal propagation, liver development, hepatocyte differentiation, cholangiocyte differentiation, multilineage differentiation, pancreatic differentiation, intestinal epithelial differentiation, cell transplantation
MeSH terms
Animals, Bile Ducts, Cell Differentiation, Cells, Cultured, Clone Cells, Epithelial Cells, Fetus, Flow Cytometry, Intestines, Liver, Liver Regeneration, Microscopy, Electron, Pancreas, Stem Cells, Biomarkers, Cell Transplantation, Proto-Oncogene Proteins c-kit, Cell Lineage, Proto-Oncogene Proteins c-met, Reverse Transcriptase Polymerase Chain Reaction, Hepatocytes, Mice

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