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Cited by 104Chromatography in Natural ProductsMolecular spectroscopy and chiralityAdvanced NMR Techniques and Applications

Hyphenated NMR methods in natural products research, part 1: Direct hyphenation

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Jaroszewski, Jerzy W.·2005·Planta Medica
DOI 10.1055/s-2005-871298
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Abstract

This review describes the current status of the development of techniques combining liquid chromatography and nuclear magnetic resonance and their use in the context of natural products. HPLC-NMR methods have a rapidly growing impact on natural products research by enabling structure determination of natural products directly from small amounts of extracts, i. e., prior to the investment in an often lengthy and laborious preparative-scale isolation process. This speeds up extract dereplication and helps to avoid re-isolation of already known extract constituents. Direct HPLC-NMR hyphenation techniques, defined as methods in which NMR spectra are recorded using the HPLC eluate, are described. The recently developed indirect HPLC-NMR method employing an automated solid-phase extraction interface between HPLC and NMR, which replaces the chromatographic solvent with a different solvent for NMR data acquisition, is described in the second part of this review.

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  • Hyphenated techniques have considerable potential in the dereplication of biological samples.

    “Although complicated and expensive to establish, hyphenated techniques have considerable potential in the dereplication of biological samples, i.e., samples can be quite rapidly screened to determine if they contain only already-known analogues or to differentiate the unknown analogues from the known ones so that time and research effort are not wasted in rediscovering compounds [143].”

    From Ecdysteroids (2021) · cites this paper as [143]

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Authors
Jaroszewski, J. W.
Corresponding author
Jaroszewski, Jerzy W.
Venue
Planta Medica
Publisher
Georg Thieme Verlag KG
Published
2005-08
Volume
71
Issue
8
Pages
691-700
ISSN
  • 0032-0943 (ISSN-L)
  • 1439-0221
DOI
10.1055/s-2005-871298
OpenAlex
W1979491221
Cited by
104
References
72
Citations by year
2025: 2 · 2024: 3 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 4 · 2018: 4 · 2017: 4 · 2016: 6 · 2015: 8 · 2014: 10 · 2013: 6 · 2012: 6
FWCI
0.96
Type
journal article
Language
de
Open access
Locations
  • Planta Medica (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
  • Physical Sciences › Chemistry › Spectroscopy › Molecular spectroscopy and chirality
  • Physical Sciences › Chemistry › Spectroscopy › Advanced NMR Techniques and Applications
Keywords
HPLC-NMR, natural products, structure elucidation, dereplication, automated solid-phase extraction, NMR data acquisition
MeSH terms
Chromatography, High Pressure Liquid, Humans, Magnetic Resonance Imaging, Phytotherapy, Plants, Medicinal

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