Agrobacterium rhizogenes-Mediated Transformation of Artemisia annua: Production of Transgenic Plants
Abstract
Transgenic hairy roots were induced in the leaves of Artemisia annua by treatment with the LBA 9402 strain of Agrobacterium rhizogenes. The axenic hairy root cultures were found to produce the sesquiterpenes artemisinic acid and arteannuin B. The hairy root cultures were observed to spontaneously regenerate into plantlets on solid hormone-free MS medium. The regenerated plants had phenotypic characteristics typical to the transformed plants. Among the plants of the age of one month in culture, the transgenic plant was bigger (2.643 g/plant) than the normal (0.856 g/plant). Both these kinds of in vitro plants carried sesquiterpenes-artemisinic acid and arteannuin B.
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Artemisinic acid and arteannuin B have been isolated from hairy root cultures of A. annua.
“These compounds have been isolated from hairy root cultures of A. annua [45].”
From Relative expression of genes of terpene metabolism in different tissues of Artemisia annuaL (2011) · cites this paper as [45]
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“These compounds have been isolated from hairy root cultures of A. annua [ 45 ].”
BackgroundMetabolic engineering of artemisinin biosynthesis in Artemisia annua L.
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“The earliest reports of successful Agrobacterium tumefaciens- and Agrobacterium rhizogenes-mediated transformation of A. annua appeared more than 10 years ago ( Banerjee et al. 1997; Vergauwe et al. 1996).”
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“Since the first reports of successful Agrobacterium-mediated transformation of A. annua in the 1990s (Vergauwe et al., 1996; Banerjee et al., 1997), these transformation protocols have been optimized (Han et al., 2005).”
BackgroundWang, J., & Tan, R. X. (2002) · Biotechnology Letters · doi:10.1023/a:1016126917795 · Cited by 55 · see citing works
“In previous studies on A. annua hairy root in flask cultures or bioreactors, MS medium characterized with higher nitrogen concentration has often been selected as the basal medium by most investigators (Qin et al. 1994, Cai et al. 1995, Banerjee et al. 1997, Liu et al. 1999).”
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“Sporadic and spontaneous regeneration of plants from hairy root cultures has also been reported in Artemisia (Banerjee et al. 1997), Tylophora (Chaudhuri et al. 2006), Pelargonium (Saxena et al. 2007), Plumbago (Gangopadhyay et al. 2010) and Ophiorrhiza (Watase et al. 2004; Kamble et al. 2011).”
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“…rhizogenes, transformation has turned out to be complex and arduous with low transformation efficiencies for this recalcitrant species (Vergauwe et al. 1996; Banerjee et al. 1997; Vergauwe et al. 1998; Chen et al. 1999, 2000; Geng et al. 2001; Teixeira da Silva 2003; Wang et al. 2004, 2007).”
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Matvieieva, N., Bohdanovych, T., & Duplij, V. P. (2023) · Slovak University of Agriculture in Nitra, Slovakia eBooks · doi:10.15414/2023.9788055226408 · Cited by 1 · see citing works
Polichuk, D. R. (2008) · University Library - University of Saskatchewan (University of Saskatchewan)
Metabolic engineering of artemisinin biosynthesis in Artemisia annua L.
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Saito, K., Muranaka, T., Sudo, H., & Kojoma, M. (2008) · Wiley eBooks · doi:10.1002/9781405181099.k0806
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- Authors
- Banerjee, S., Zehra, M., Gupta, M., & Kumar, S.
- Affiliations
- Central Institute of Medicinal and Aromatic Plants (IN)
- Venue
- Planta Medica
- Publisher
- Georg Thieme Verlag KG
- Published
- 1997-10
- Volume
- 63
- Issue
- 05
- Pages
- 467-469
- ISSN
- 0032-0943 (ISSN-L)
- 1439-0221
- OpenAlex
- W2014709803
- Cited by
- 31
- References
- 0
- Citations by year
- 2024: 1 · 2023: 1 · 2020: 6 · 2018: 1 · 2016: 1 · 2015: 1 · 2014: 3 · 2013: 6
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Planta Medica (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Keywords
- Artemisia annua, Agrobacterium rhizogenes, hairy roots, transgenic plants, artemisinic acid, arteannuin B, sesquiterpenes, natural regeneration, plant growth
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