The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Mutational Activation of the K-ras Oncogene
Abstract
To define the role of cellular oncogenes in human cancers, we studied the prevalence of mutational activation of ras oncogenes in untreated non-small-cell lung cancer. Genomic DNA was extracted from 39 tumor specimens obtained by thoracotomy and was examined for activating point mutations in codons 12, 13, and 61 of the H-ras, K-ras, and N-ras genes. A novel, highly sensitive assay based on oligonucleotide hybridization following an in vitro amplification step was employed. The K-ras gene was found to be activated by point mutations in codon 12 in 5 of 10 adenocarcinomas. Two of these tumors were less than 2 cm in size and had not metastasized. No ras gene mutations were observed in 15 squamous-cell carcinomas, 10 large-cell carcinomas, 1 carcinoid, 2 metastatic adenocarcinomas from primary tumors outside the lung, and 1 small-cell carcinoma. An approximately 20-fold amplification of the unmutated K-ras gene was observed in a tumor that proved to be a solitary lung metastasis of a rectal carcinoma. We conclude that mutational K-ras activation may be an important early event in the pathogenesis of adenocarcinoma of the lung but that amplification of ras genes or mutational activation of H-ras or N-ras does not play a major part in non-small-cell lung cancer.
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Cites
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Cited by
Epidermal growth factor receptor mutations in lung cancer
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Mapping the Hallmarks of Lung Adenocarcinoma with Massively Parallel Sequencing
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Metadata
- Authors
- Rodenhuis, S., van de Wetering, M. L., Mooi, W. J., Evers, S. G., van Zandwijk, N., & Bos, J. L.
- Venue
- New England Journal of Medicine
- Publisher
- Massachusetts Medical Society
- Published
- 1987-10-08
- Volume
- 317
- Issue
- 15
- Pages
- 929-935
- ISSN
- 0028-4793 (ISSN-L)
- 1533-4406
- OpenAlex
- W2320138981
- Cited by
- 536
- References
- 31
- FWCI
- 46.98
- Type
- journal article
- Language
- en
- Open access
- closed
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