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The Agent of Bacillary Angiomatosis
Abstract
Abstract Background. Bacillary angiomatosis is an infectious disease causing proliferation of small blood vessels in the skin and visceral organs of patients with human immunodeficiency virus infection and other immunocompromised hosts. The agent is often visualized in tissue sections of lesions with Warthin—Starry staining, but the bacillus has not been successfully cultured or identified. This bacillus may also cause cat scratch disease. Methods. In attempting to identify this organism, we used the polymerase chain reaction. We used oligonucleotide primers complementary to the 16S ribosomal RNA genes of eubacteria to amplify 16S ribosomal gene fragments directly from tissue samples of bacillary angiomatosis. The DNA sequence of these fragments was determined and analyzed for phylogenetic relatedness to other known organisms. Normal tissues were studied in parallel. Results. Tissue from three unrelated patients with bacillary angiomatosis yielded a unique 16S gene sequence. A sequence obtained from a fou...
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The Agent of Bacillary Angiomatosis: An Approach to the Identification of Uncultured Pathogens
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Epidemiology and causes of preterm birth
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Molecular diagnosis of bacterial endocarditis by broad-range PCR amplification and direct sequencing
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Metadata
- Venue
- New England Journal of Medicine
- Publisher
- Massachusetts Medical Society
- Published
- 1991-05-23
- Volume
- 324
- Issue
- 21
- Pages
- 1511-1513
- ISSN
- 0028-4793 (ISSN-L)
- 1533-4406
- OpenAlex
- W2337345147
- Cited by
- 290
- References
- 1
- FWCI
- 11.29
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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