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Cited by 19Microfluidic and Bio-sensing TechnologiesMicrofluidic and Capillary Electrophoresis ApplicationsCellular Mechanics and Interactions

Modulating chemotaxis of lung cancer cells by using electric fields in a microfluidic device

Open access
Kao, Yu-Chiu, Hsieh, Meng-Hua, Liu, Chung-Chun, Pan, Huei-Jyuan, Liao, Wei-Yu, Cheng, Ji-Yen, Kuo, Po-Ling, Lee, Chau-Hwang·2014·Biomicrofluidics
DOI 10.1063/1.4870401PMC PMC3976467
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Abstract

We employed direct-current electric fields (dcEFs) to modulate the chemotaxis of lung cancer cells in a microfluidic cell culture device that incorporates both stable concentration gradients and dcEFs. We found that the chemotaxis induced by a 0.5 μM/mm concentration gradient of epidermal growth factor can be nearly compensated by a 360 mV/mm dcEF. When the effect of chemical stimulation was balanced by the electrical drive, the cells migrated randomly, and the path lengths were largely reduced. We also demonstrated electrically modulated chemotaxis of two types of lung cancer cells with opposite directions of electrotaxis in this device.

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Authors
Kao, Y.-C., Hsieh, M.-H., Liu, C.-C., Pan, H.-J., Liao, W.-Y., Cheng, J.-Y., Kuo, P.-L., & Lee, C.-H.
Affiliations
  • National Taiwan University (TW)
  • National Yang Ming University (TW)
  • Research Center for Applied Science, Academia Sinica (TW)
  • National Taiwan University Hospital (TW)
ORCID
Venue
Biomicrofluidics
Publisher
AIP Publishing
Published
2014-03-01
Volume
8
Issue
2
Pages
024107-024107
ISSN
1932-1058 (ISSN-L)
DOI
10.1063/1.4870401
PMC
PMC3976467
OpenAlex
W1992982533
Cited by
19
References
32
Citations by year
2024: 2 · 2023: 1 · 2021: 1 · 2019: 3 · 2017: 3 · 2016: 6 · 2015: 2 · 2014: 1
FWCI
2.08
Type
journal article
Language
en
Open access
Locations
  • Biomicrofluidics (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Science Council (NSC-100-2112-M-001-022-MY3))
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
  • Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
lung cancer cells, chemotaxis, electrotaxis, DC electric field, microfluidic cell culture, epidermal growth factor, concentration gradient, cell migration, random migration

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