The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Cytokine detection by antibody-based proximity ligation
Open accessAbstract
Efficient and precise detection techniques, along with extensive repertoires of specific binding reagents, will be needed to meet the challenges of proteome analyses. The recently established proximity ligation mechanism enables sensitive high-capacity protein measurements by converting the detection of specific proteins to the analysis of DNA sequences. Proximity probes containing oligonucleotide extensions are designed to bind pairwise to target proteins and to form amplifiable tag sequences by ligation when brought in proximity. In our previous report, both the ligatable arms and the protein binders were DNA molecules. We now generalize the method by providing simple and convenient protocols to convert any polyclonal antibodies or matched pair of monoclonal antibodies to proximity probe sets through the attachment of oligonucleotide sequences. Sufficient reagent for >100,000 proximity ligation assays can be prepared from 1 μg of antibody. The technique is applied to measure cytokines in a homogenous test format with femtomolar detection sensitivities in 1-μl samples, and we exemplify its utility in situations when only minute sample amounts are available.
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Cites
Protein detection using proximity-dependent DNA ligation assays
Fredriksson, S., Gullberg, M., Jarvius, J., Olsson, C., Pietras, K., Gústafsdóttir, S. M., Östman, A., & Landegren, U. (2002) · Nature Biotechnology · doi:10.1038/nbt0502-473 · Cited by 1,504
Self-citationImmuno-PCR: Very Sensitive Antigen Detection by Means of Specific Antibody-DNA Conjugates
Sano, T., Smith, C. L., & Cantor, C. R. (1992) · Science · doi:10.1126/science.1439758 · Cited by 861
Oligonucleotide inhibitors of human thrombin that bind distinct epitopes
Tasset, D. M., Kubik, M. F., & Steiner, W. (1997) · Journal of Molecular Biology · doi:10.1006/jmbi.1997.1275 · Cited by 837
Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity
Boder, E. T., Midelfort, K. S., & Wittrup, K. D. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.170297297 · Cited by 673
Endothelial PDGF-B retention is required for proper investment of pericytes in the microvessel wall
Lindblom, P., Gerhardt, H., Liebner, S., Abramsson, A., Enge, M., Hellström, M., Bäckström, G., Fredriksson, S., Landegren, U., Nyström, H. C., et al. (2003) · Genes & Development · doi:10.1101/gad.266803 · Cited by 657
Self-citationChen, Y., Wiesmann, C., Fuh, G., Li, B., Christinger, H. W., McKay, P., de Vos, A. M., & Lowman, H. B. (1999) · Journal of Molecular Biology · doi:10.1006/jmbi.1999.3192 · Cited by 448
Inhibitory DNA Ligands to Platelet-Derived Growth Factor B-Chain
Green, L. S., Jellinek, D., Jenison, R., Östman, A., Heldin, C.-H., & Janjic, N. (1996) · Biochemistry · doi:10.1021/bi961544+ · Cited by 390
Hanes, J., Schaffitzel, C., Knappik, A., & Plückthun, A. (2000) · Nature Biotechnology · doi:10.1038/82407 · Cited by 373
Bjarnegård, M., Enge, M., Norlin, J., Gustafsdottir, S., Fredriksson, S., Abramsson, A., Takemoto, M., Gustafsson, E., Fässler, R., & Betsholtz, C. (2004) · Development · doi:10.1242/dev.01080 · Cited by 369
Self-citationSelf-assembly of DNA-streptavidin nanostructures and their use as reagents in immuno-PCR
Niemeyer, C. (1999) · Nucleic Acids Research · doi:10.1093/nar/27.23.4553 · Cited by 211
Cited by
Direct observation of individual endogenous protein complexes in situ by proximity ligation
Söderberg, O., Gullberg, M., Jarvius, M., Ridderstråle, K., Leuchowius, K.-J., Jarvius, J., Wester, K., Hydbring, P., Bahram, F., Larsson, L.-G., et al. (2006) · Nature Methods · doi:10.1038/nmeth947 · Cited by 2,419
Self-citationAssarsson, E., Lundberg, M., Holmquist, G., Björkesten, J., Bucht Thorsen, S., Ekman, D., Eriksson, A., Rennel Dickens, E., Ohlsson, S., Edfeldt, G., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0095192 · Cited by 1,874
Self-citationFunctional Aptamers and Aptazymes in Biotechnology, Diagnostics, and Therapy
Famulok, M., Hartig, J. S., & Mayer, G. (2007) · Chemical Reviews · doi:10.1021/cr0306743 · Cited by 887
Lundberg, M., Eriksson, A., Tran, B., Assarsson, E., & Fredriksson, S. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr424 · Cited by 744
Self-citationDeciphering molecular interactions by proximity labeling
Qin, W., Cho, K. F., Cavanagh, P. E., & Ting, A. Y. (2021) · Nature Methods · doi:10.1038/s41592-020-01010-5 · Cited by 671
Metadata
- Authors
- Gullberg, M., Gústafsdóttir, S. M., Schallmeiner, E., Jarvius, J., Bjarnegård, M., Betsholtz, C., Landegren, U., & Fredriksson, S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-05-21
- Volume
- 101
- Issue
- 22
- Pages
- 8420-8424
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1977093496
- Cited by
- 411
- References
- 16
- FWCI
- 4.37
- Type
- journal article
- Language
- en
- Open access
- green
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