The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Biological activity of nitric oxide in the plasmatic compartment
Open accessAbstract
There exist reaction products of nitric oxide (NO) with blood that conserve its bioactivity and transduce an endocrine vasomotor function under certain conditions. Although S-nitrosated albumin has been considered the major species subserving this activity, recent data suggest that additional NO species, such as nitrite, nitrated lipids, N-nitrosamine, and iron-nitrosyl complexes, may contribute. We therefore examined the end products of NO reactions in plasma and blood in vitro and in vivo by using reductive chemiluminescent assays and electron paramagnetic resonance spectroscopy. We found that NO complexes in plasma previously considered to be S-nitrosated albumin were <10 nM after elimination of nitrite and were mercury-stable, consistent with iron-nitrosyl or N-nitrosamine complex. During clinical NO gas inhalation protocols or in vitro NO donor treatment of human plasma, S-nitroso-albumin did not form with NO exposure <2 microM, but plasma methemoglobin was detectable by paramagnetic resonance spectroscopy. Consistent with this formation of methemoglobin, human plasma was found to consume approximately 2 microM NO at a rate equivalent to that of hemoglobin. This NO consumption was mediated by the reaction of NO with plasma haptoglobin-hemoglobin complexes and limited slower reaction pathways required for S-nitrosation. These data suggest that high-affinity, metal-based reactions in plasma with the haptoglobin-hemoglobin complex modulate plasmatic NO reaction products and limit S-nitrosation at low NO flux. The studies further suggest that alternative NO reaction end products in plasma, such as nitrite, N-nitrosamines, iron-nitrosyls, and nitrated lipids, should be evaluated in blood NO transport along the vasculature.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“It was also shown that the addition of NO to blood generates significant concentrations of plasma nitrite that involved copper oxidation, which was found to be ceruloplasmin dependent NO oxidation [78, 79].”
From Inorganic nitrite therapy: historical perspective and future directions (2011) · cites this paper as [79]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation
Cosby, K., Partovi, K. S., Crawford, J. H., Patel, R. P., Reiter, C. D., Martyr, S., Yang, B. K., Waclawiw, M. A., Zalos, G., Xu, X., et al. (2003) · Nature Medicine · doi:10.1038/nm954 · Cited by 1,807
Self-citationStamler, J. S., Simon, D. I., Osborne, J. A., Mullins, M. E., Jaraki, O., Michel, T. M., Singel, D. J., & Loscalzo, J. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.1.444 · Cited by 1,471
Cell-free hemoglobin limits nitric oxide bioavailability in sickle-cell disease
Reiter, C. D., Wang, X., Tanus-Santos, J. E., Hogg, N., Cannon, R. O., Schechter, A. N., & Gladwin, M. T. (2002) · Nature Medicine · doi:10.1038/nm1202-799 · Cited by 1,330
Self-citationNitric oxide circulates in mammalian plasma primarily as an S-nitroso adduct of serum albumin.
Stamler, J. S., Jaraki, O., Osborne, J., Simon, D. I., Keaney, J., Vita, J., Singel, D., Valeri, C. R., & Loscalzo, J. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.16.7674 · Cited by 1,238
Cell-free hemoglobin limits nitric oxide bioavailability in sickle-cell disease
Reiter, C. D., Wang, X., Tanus-Santos, J. E., et al. (2002) · Nature Medicine · doi:10.1038/nm799 · Cited by 1,097
Self-citationThe biological lifetime of nitric oxide: Implications for the perivascular dynamics of NO and O 2
Thomas, D. D., Liu, X., Kantrow, S. P., & Lancaster, J. R. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.98.1.355 · Cited by 760
Diffusion-limited reaction of free nitric oxide with erythrocytes
Liu, X., Miller, M. J. S., Joshi, M. S., Sadowska-Krowicka, H., Clark, D. A., & Lancaster, J. R. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.30.18709 · Cited by 512
Gladwin, M. T., Shelhamer, J. H., Schechter, A. N., Pease-Fye, M. E., Waclawiw, M. A., Panza, J. A., Ognibene, F. P., & Cannon, R. O. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.21.11482 · Cited by 441
Self-citationThrombin Activates the Hypoxia-Inducible Factor-1 Signaling Pathway in Vascular Smooth Muscle Cells
Görlach, A., Diebold, I., Schini‐Kerth, V. B., Berchner‐Pfannschmidt, U., Roth, U., Brandes, R. P., Kietzmann, T., & Busse, R. (2001) · Circulation Research · doi:10.1161/hh1301.092678 · Cited by 413
Intravascular flow decreases erythrocyte consumption of nitric oxide
Liao, J. C., Hein, T. W., Vaughn, M. W., Huang, K., & Kuo, L. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.15.8757 · Cited by 301
Research works printed but not linked: 4
- Gruetter, C. A., Barry, B. K., McNamara, D. B., Kadowitz, P. J. & Ignarro, L. J. (1980) J. Pharmacol. Exp. Ther. 214, 9-15.6104719
- Joshi, M. S., Ferguson, T. B., Jr., Han, T. H., Hyduke, D. R., Liao, J. C., Rassaf, T., Bryan, N., Feelisch, M. & Lancaster, J. R., Jr. (2002) Proc. Natl. Acad. Sci. USA 17, 17.
- Gladwin, M. T., Wang, X., Reiter, C. D., Yang, B. K., Vivas, E. X., Bonaventura, C. & Schechter, A. N. (2002) J. Biol. Chem. 21, 21.
- Joshi, M. S., Ferguson, T. B., Jr., Han, T. H., Hyduke, D. R., Liao, J. C., Rassaf, T., Bryan, N., Feelisch, M. & Lancaster, J. R., Jr. (2002) Proc. Natl. Acad. Sci. USA 17, 10341-10346.
Cited by
Sorted by citations
Quinlan, G. J., Martin, G. S., & Evans, T. W. (2005) · Hepatology · doi:10.1002/hep.20720 · Cited by 985
Morris, C. R. (2005) · JAMA · doi:10.1001/jama.294.1.81 · Cited by 769
Formal guidelines: Management of acute respiratory distress syndrome.
Papazian, L., Aubron, C., Brochard, L., Chiche, J.-D., Combes, A., Dreyfuss, D., Forel, J.-M., Guérin, C., Jaber, S., Mekontso-Dessap, A., et al. (2019) · Annals of Intensive Care · doi:10.1186/s13613-019-0540-9 · Cited by 761
Donadee, C., Raat, N. J. H., Kanias, T., Tejero, J., Lee, J. S., Kelley, E. E., Zhao, X., Liu, C., Reynolds, H., Azarov, I., et al. (2011) · Circulation · doi:10.1161/circulationaha.110.008698 · Cited by 584
Self-citationSingel, D. J., & Stamler, J. S. (2005) · Annual Review of Physiology · doi:10.1146/annurev.physiol.67.060603.090918 · Cited by 481
Metadata
- Authors
- Wang, X., Tanus-Santos, J. E., Reiter, C. D., Dejam, A., Shiva, S., Smith, R. D., Hogg, N., & Gladwin, M. T.
- Affiliations
- National Institutes of Health (US)
- Critical Care Medicine Department, Warren G. Magnuson Clinical Center, Laboratory of Chemical Biology, and Molecular and Clinical Hematology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, and Cardiovascular Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1662; and Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226
- Corresponding author
- Gladwin, Mark T.
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2004-07-16
- Volume
- 101
- Issue
- 31
- Pages
- 11477-11482
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC509225
- OpenAlex
- W2081108089
- Cited by
- 164
- References
- 49
- Citations by year
- 2026: 1 · 2024: 5 · 2023: 3 · 2022: 5 · 2021: 2 · 2020: 2 · 2019: 7 · 2018: 2 · 2017: 4 · 2016: 5 · 2015: 6 · 2014: 5 · 2013: 9 · 2012: 15
- FWCI
- 10.48
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Hemoglobin structure and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- nitric oxide, plasma nitric oxide, S-nitrosylation, iron nitrosyl complexes, nitrosamines, nitrite, hemoglobin-haptoglobin complex, methemoglobin, nitric oxide donors, vasomotor function
- MeSH terms
- Administration, Inhalation, Haptoglobins, Hemoglobins, Humans, Iron, Methemoglobin, Nitric Oxide, Nitrites, Plasma, Serum Albumin, S-Nitrosothiols, In Vitro Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
