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Cited by 892

The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.

Complete genomes of two clinical Staphylococcus aureus strains: Evidence for the rapid evolution of virulence and drug resistance

Open access
Holden, Matthew T. G., Feil, Edward J., Lindsay, Jodi A., Peacock, Sharon J., Day, Nicholas P. J., Enright, Mark C., Foster, Tim J., Moore, Catrin E., Hurst, Laurence, Atkin, Rebecca, Barron, Andrew, Bason, Nathalie, Bentley, Stephen D., Chillingworth, Carol, Chillingworth, Tracey, Churcher, Carol, Clark, Louise, Corton, Craig, Cronin, Ann, Doggett, Jon, Dowd, Linda, Feltwell, Theresa, Hance, Zahra, Harris, Barbara, Hauser, Heidi, Holroyd, Simon, Jagels, Kay, James, Keith D., Lennard, Nicola, Line, Alexandra, Mayes, Rebecca, Moule, Sharon, Mungall, Karen, Ormond, Douglas, Quail, Michael A., Rabbinowitsch, Ester, Rutherford, Kim, Sanders, Mandy, Sharp, Sarah, Simmonds, Mark, Stevens, Kim, Whitehead, Sally, Barrell, Bart G., Spratt, Brian G., Parkhill, Julian·2004·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.0402521101
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Abstract

Staphylococcus aureus is an important nosocomial and community-acquired pathogen. Its genetic plasticity has facilitated the evolution of many virulent and drug-resistant strains, presenting a major and constantly changing clinical challenge. We sequenced the ≈2.8-Mbp genomes of two disease-causing S. aureus strains isolated from distinct clinical settings: a recent hospital-acquired representative of the epidemic methicillin-resistant S. aureus EMRSA-16 clone (MRSA252), a clinically important and globally prevalent lineage; and a representative of an invasive community-acquired methicillin-susceptible S. aureus clone (MSSA476). A comparative-genomics approach was used to explore the mechanisms of evolution of clinically important S. aureus genomes and to identify regions affecting virulence and drug resistance. The genome sequences of MRSA252 and MSSA476 have a well conserved core region but differ markedly in their accessory genetic elements. MRSA252 is the most genetically diverse S. aureus strain sequenced to date: ≈6% of the genome is novel compared with other published genomes, and it contains several unique genetic elements. MSSA476 is methicillin-susceptible, but it contains a novel Staphylococcal chromosomal cassette (SCC) mec -like element (designated SCC 476 ), which is integrated at the same site on the chromosome as SCC mec elements in MRSA strains but encodes a putative fusidic acid resistance protein. The crucial role that accessory elements play in the rapid evolution of S. aureus is clearly illustrated by comparing the MSSA476 genome with that of an extremely closely related MRSA community-acquired strain; the differential distribution of large mobile elements carrying virulence and drug-resistance determinants may be responsible for the clinically important phenotypic differences in these strains.

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Metadata

Authors
Holden, M. T. G., Feil, E. J., Lindsay, J. A., Peacock, S. J., Day, N. P. J., Enright, M. C., Foster, T. J., Moore, C. E., Hurst, L., Atkin, R., Barron, A., Bason, N., Bentley, S. D., Chillingworth, C., Chillingworth, T., Churcher, C., Clark, L., Corton, C., Cronin, A., Doggett, J., Dowd, L., Feltwell, T., Hance, Z., Harris, B., Hauser, H., Holroyd, S., Jagels, K., James, K. D., Lennard, N., Line, A., Mayes, R., Moule, S., Mungall, K., Ormond, D., Quail, M. A., Rabbinowitsch, E., Rutherford, K., Sanders, M., Sharp, S., Simmonds, M., Stevens, K., Whitehead, S., Barrell, B. G., Spratt, B. G., & Parkhill, J.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
2004-06-22
Volume
101
Issue
26
Pages
9786-9791
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.0402521101
OpenAlex
W2113651812
Cited by
892
References
30
FWCI
37.70
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Complete genomes of two clinical Staphylococcus aureus strains: Evidence for the rapid evolution of virulence and drug resistance. https://app.cochat.ai/papers/10.1073/pnas.0402521101
The paper
Holden, M. T. G., Feil, E. J., Lindsay, J. A., Peacock, S. J., Day, N. P. J., Enright, M. C., Foster, T. J., Moore, C. E., Hurst, L., Atkin, R., Barron, A., Bason, N., Bentley, S. D., Chillingworth, C., Chillingworth, T., Churcher, C., Clark, L., Corton, C., Cronin, A., . . . Parkhill, J. (2004). Complete genomes of two clinical Staphylococcus aureus strains: Evidence for the rapid evolution of virulence and drug resistance. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0402521101

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.