The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Programmable cells: Interfacing natural and engineered gene networks
Open accessAbstract
Novel cellular behaviors and characteristics can be obtained by coupling engineered gene networks to the cell's natural regulatory circuitry through appropriately designed input and output interfaces. Here, we demonstrate how an engineered genetic circuit can be used to construct cells that respond to biological signals in a predetermined and programmable fashion. We employ a modular design strategy to create Escherichia coli strains where a genetic toggle switch is interfaced with: ( i ) the SOS signaling pathway responding to DNA damage, and ( ii ) a transgenic quorum sensing signaling pathway from Vibrio fischeri. The genetic toggle switch endows these strains with binary response dynamics and an epigenetic inheritance that supports a persistent phenotypic alteration in response to transient signals. These features are exploited to engineer cells that form biofilms in response to DNA-damaging agents and cells that activate protein synthesis when the cell population reaches a critical density. Our work represents a step toward the development of “plug-and-play” genetic circuitry that can be used to create cells with programmable behaviors.
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Cites
Stochastic Gene Expression in a Single Cell
Elowitz, M. B., Levine, A. J., Siggia, E. D., & Swain, P. S. (2002) · Science · doi:10.1126/science.1070919 · Cited by 5,441
Inferring Genetic Networks and Identifying Compound Mode of Action via Expression Profiling
Gardner, T. S., di Bernardo, D., Lorenz, D., & Collins, J. J. (2003) · Science · doi:10.1126/science.1081900 · Cited by 1,117
Self-citationMultistability in the lactose utilization network of Escherichia coli
Ozbudak, E. M., Thattai, M., Lim, H. N., Shraiman, B. I., & van Oudenaarden, A. (2004) · Nature · doi:10.1038/nature02298 · Cited by 1,030
Motifs, modules and games in bacteria
Wolf, D. M., & Arkin, A. P. (2003) · Current Opinion in Microbiology · doi:10.1016/s1369-5274(03)00033-x · Cited by 303
Cited by
Synthetic biology: new engineering rules for an emerging discipline
Andrianantoandro, E., Basu, S., Karig, D. K., & Weiss, R. (2006) · Molecular Systems Biology · doi:10.1038/msb4100073 · Cited by 1,081
A synchronized quorum of genetic clocks
Danino, T., Mondragón-Palomino, O., Tsimring, L., & Hasty, J. (2010) · Nature · doi:10.1038/nature08753 · Cited by 992
Synthetic Gene Networks That Count
Friedland, A. E., Lu, T. K., Wang, X., Shi, D., Church, G., & Collins, J. J. (2009) · Science · doi:10.1126/science.1172005 · Cited by 587
Self-citationA synthetic Escherichia coli predator–prey ecosystem
Balagaddé, F. K., Song, H., Ozaki, J., Collins, C. H., Barnet, M., Arnold, F. H., Quake, S. R., & You, L. (2008) · Molecular Systems Biology · doi:10.1038/msb.2008.24 · Cited by 482
Isaacs, F. J., Dwyer, D. J., & Collins, J. J. (2006) · Nature Biotechnology · doi:10.1038/nbt1208 · Cited by 373
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Metadata
- Authors
- Kobayashi, H., Kærn, M., Araki, M., Chung, K., Gardner, T. S., Cantor, C. R., & Collins, J. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-05-24
- Volume
- 101
- Issue
- 22
- Pages
- 8414-8419
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2111870329
- Cited by
- 602
- References
- 4
- FWCI
- 11.92
- Type
- journal article
- Language
- en
- Open access
- green
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