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Poly-β amino ester-containing microparticles enhance the activity of nonviral genetic vaccines
Open accessAbstract
Current nonviral genetic vaccine systems are less effective than viral vaccines, particularly in cancer systems where epitopes can be weakly immunogenic and antigen-presenting cell processing and presentation to T cells is down-regulated. A promising nonviral delivery method for genetic vaccines involves microencapsulation of antigen-encoding DNA, because such particles protect plasmid payloads and target them to phagocytic antigen-presenting cells. However, conventional microparticle formulations composed of poly lactic-co-glycolic acid take too long to release encapsulated payload and fail to induce high levels of target gene expression. Here, we describe a microparticle-based DNA delivery system composed of a degradable, pH-sensitive poly-β amino ester and poly lactic-co-glycolic acid. These formulations generate an increase of 3–5 orders of magnitude in transfection efficiency and are potent activators of dendritic cells in vitro. When used as vaccines in vivo, these microparticle formulations, unlike conventional formulations, induce antigen-specific rejection of transplanted syngenic tumor cells.
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Cited by
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Tian, H., Tang, Z., Zhuang, X., Chen, X., & Jing, X. (2012) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2011.06.004 · Cited by 1,293
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Sun, C.-Y., Qin, C., Wang, X.-L., Yang, G.-S., Shao, K.-Z., Lan, Y.-Q., Su, Z.-M., Huang, P., Wang, C.-G., & Wang, E.-B. (2012) · Dalton Transactions · doi:10.1039/c2dt30357d · Cited by 710
Turning the corner on therapeutic cancer vaccines
Hollingsworth, R. E., & Jansen, K. (2019) · npj Vaccines · doi:10.1038/s41541-019-0103-y · Cited by 689
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Green, J. J., Langer, R., & Anderson, D. G. (2008) · Accounts of Chemical Research · doi:10.1021/ar7002336 · Cited by 579
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Metadata
- Authors
- Little, S. R., Lynn, D. M., Ge, Q., Anderson, D. G., Puram, S. V., Chen, J., Eisen, H. N., & Langer, R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-06-21
- Volume
- 101
- Issue
- 26
- Pages
- 9534-9539
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2170390976
- Cited by
- 227
- References
- 0
- FWCI
- 5.00
- Type
- journal article
- Language
- en
- Open access
- green
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- Little, S. R., Lynn, D. M., Ge, Q., Anderson, D. G., Puram, S. V., Chen, J., Eisen, H. N., & Langer, R. (2004). Poly-β amino ester-containing microparticles enhance the activity of nonviral genetic vaccines. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0403549101
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