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Cited by 347

The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.

Muscle-specific RING finger 1 is a bona fide ubiquitin ligase that degrades cardiac troponin I

Open access
Kedar, Vishram, McDonough, Holly, Arya, Ranjana, Li, Hui-Hua, Rockman, Howard A., Patterson, Cam·2004·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.0404341102
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Abstract

Muscle-specific RING finger protein 1 (MuRF1) is a sarcomere-associated protein that is restricted to cardiac and skeletal muscle. In skeletal muscle, MuRF1 is up-regulated by conditions that provoke atrophy, but its function in the heart is not known. The presence of a RING finger in MuRF1 raises the possibility that it is a component of the ubiquitin–proteasome system of protein deg-radation. We performed a yeast two-hybrid screen to search for interaction partners of MuRF1 in the heart that might be targets of its putative ubiquitin ligase activity. This screen identified troponin I as a MuRF1 partner protein. MuRF1 and troponin I were found to associate both in vitro and in vivo in cultured cardiomyocytes. MuRF1 reduced steady-state troponin I levels when coexpressed in COS-7 cells and increased degradation of endogenous troponin I protein in cardiomyocytes. The degradation of troponin I in cardiomyocytes was associated with the accumulation of ubiquitylated intermediates of troponin I and was proteasome-dependent. In vitro , MuRF1 functioned as a ubiquitin ligase to catalyze ubiquitylation of troponin I through a RING finger-dependent mechanism. In isolated cardiomyocytes, MuRF1 reduced indices of contractility. In cardiomyocytes, these processes may determine the balance between hypertrophic and antihypertrophic signals and the regulation of myocyte contractile responses in the setting of heart failure.

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Metadata

Authors
Kedar, V., McDonough, H., Arya, R., Li, H.-H., Rockman, H. A., & Patterson, C.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
2004-12-15
Volume
101
Issue
52
Pages
18135-18140
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.0404341102
OpenAlex
W1992427679
Cited by
347
References
34
FWCI
6.23
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Muscle-specific RING finger 1 is a bona fide ubiquitin ligase that degrades cardiac troponin I. https://app.cochat.ai/papers/10.1073/pnas.0404341102
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Kedar, V., McDonough, H., Arya, R., Li, H.-H., Rockman, H. A., & Patterson, C. (2004). Muscle-specific RING finger 1 is a bona fide ubiquitin ligase that degrades cardiac troponin I. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0404341102

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.