The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
FRET measurements of cell-traction forces and nano-scale clustering of adhesion ligands varied by substrate stiffness
Open accessAbstract
The mechanical properties of cell adhesion substrates regulate cell phenotype, but the mechanism of this relation is currently unclear. It may involve the magnitude of traction force applied by the cell, and/or the ability of the cells to rearrange the cell adhesion molecules presented from the material. In this study, we describe a FRET technique that can be used to evaluate the mechanics of cell–material interactions at the molecular level and simultaneously quantify the cell-based nanoscale rearrangement of the material itself. We found that these events depended on the mechanical rigidity of the adhesion substrate. Furthermore, both the proliferation and differentiation of preosteoblasts (MC3T3-E1) correlated to the magnitude of force that cells generate to cluster the cell adhesion ligands, but not the extent of ligand clustering. Together, these data demonstrate the utility of FRET in analyzing cell–material interactions, and suggest that regulation of phenotype with substrate stiffness is related to alterations in cellular traction forces.
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Cites
Taking Cell-Matrix Adhesions to the Third Dimension
Cukierman, E., Pankov, R., Stevens, D. R., & Yamada, K. M. (2001) · Science · doi:10.1126/science.1064829 · Cited by 3,027
Myotubes differentiate optimally on substrates with tissue-like stiffness
Engler, A. J., Griffin, M. A., Sen, S., Bönnemann, C. G., Sweeney, H. L., & Discher, D. E. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200405004 · Cited by 1,743
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Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
Hydrogels with tunable stress relaxation regulate stem cell fate and activity
Chaudhuri, O., Gu, L., Klumpers, D., Darnell, M., Bencherif, S. A., Weaver, J. C., Huebsch, N., Lee, H.-P., Lippens, E., Duda, G. N., et al. (2016) · Nature Materials · doi:10.1038/nmat4489 · Cited by 2,443
Self-citationMechanotransduction and extracellular matrix homeostasis
Humphrey, J. D., Dufresne, E. R., & Schwartz, M. A. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3896 · Cited by 2,124
Three-Dimensional Cell Culture Matrices: State of the Art
Lee, J., Cuddihy, M. J., & Kotov, N. A. (2008) · Tissue Engineering Part B: Reviews · doi:10.1089/teb.2007.0150 · Cited by 1,047
Designing degradable hydrogels for orthogonal control of cell microenvironments
Kharkar, P. M., Kiick, K. L., & Kloxin, A. M. (2013) · Chem. Soc. Rev. · doi:10.1039/c3cs60040h · Cited by 717
Metadata
- Authors
- Kong, H. J., Polte, T. R., Alsberg, E., & Mooney, D. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2005-03-14
- Volume
- 102
- Issue
- 12
- Pages
- 4300-4305
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2000000908
- Cited by
- 308
- References
- 2
- FWCI
- 6.68
- Type
- journal article
- Language
- en
- Open access
- green
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- Kong, H. J., Polte, T. R., Alsberg, E., & Mooney, D. J. (2005). FRET measurements of cell-traction forces and nano-scale clustering of adhesion ligands varied by substrate stiffness. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0405873102
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