The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Bursicon, the insect cuticle-hardening hormone, is a heterodimeric cystine knot protein that activates G protein-coupled receptor LGR2
Open accessAbstract
All arthropods periodically molt to replace their exoskeleton (cuticle). Immediately after shedding the old cuticle, the neurohormone bursicon causes the hardening and darkening of the new cuticle. Here we show that bursicon, to our knowledge the first heterodimeric cystine knot hormone found in insects, consists of two proteins encoded by the genes burs and pburs ( partner of burs ). The pburs/burs heterodimer from Drosophila melanogaster binds with high affinity and specificity to activate the G protein-coupled receptor DLGR2, leading to the stimulation of cAMP signaling in vitro and tanning in neck-ligated blowflies. Native bursicon from Periplaneta americana is also a heterodimer. In D. melanogaster the levels of pburs , burs , and DLGR2 transcripts are increased before ecdysis, consistent with their role in postecdysial cuticle changes. Immunohistochemical analyses in diverse insect species revealed the colocalization of pburs- and burs-immunoreactivity in some of the neurosecretory neurons that also express crustacean cardioactive peptide. Forty-three years after its initial description, the elucidation of the molecular identity of bursicon and the verification of its receptor allow for studies of bursicon actions in regulating cuticle tanning, wing expansion, and as yet unknown functions. Because bursicon subunit genes are homologous to the vertebrate bone morphogenetic protein antagonists, our findings also facilitate investigation on the function of these proteins during vertebrate development.
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Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, S. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.17.3389 · Cited by 73,302
Bone Morphogenetic Proteins, Their Antagonists, and the Skeleton
Canalis, E., Economides, A. N., & Gazzerro, E. (2003) · Endocrine Reviews · doi:10.1210/er.2002-0023 · Cited by 863
Activation of Orphan Receptors by the Hormone Relaxin
Hsu, S. Y., Nakabayashi, K., Nishi, S., Kumagai, J., Kudo, M., Sherwood, O. D., & Hsueh, A. J. W. (2002) · Science · doi:10.1126/science.1065654 · Cited by 786
Self-citationEcdysone : from chemistry to mode of action
Koolman, J., Becker, J., & Karlson, P. (1989) · Medical Entomology and Zoology · Cited by 490
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Kumagai, J., Hsu, S. Y., Matsumi, H., Roh, J.-S., Fu, P., Wade, J. D., Bathgate, R. A. D., & Hsueh, A. J. W. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.c200398200 · Cited by 407
Self-citationHsueh, A. J. W., 工藤, 正., Chen, T., Nakabayashi, K., Bhalla, A., van der Spek, P. J., van Duin, M., & Hsueh, A. J. W. (2000) · Molecular Endocrinology · doi:10.1210/me.14.8.1257 · Cited by 358
Self-citationVitt, U. A., Hsueh, A. J. W., & Hsueh, A. J. W. (2001) · Molecular Endocrinology · doi:10.1210/me.15.5.681 · Cited by 304
Self-citationAvsian-Kretchmer, O., & Hsueh, A. J. W. (2004) · Molecular Endocrinology · doi:10.1210/me.2003-0227 · Cited by 229
Self-citationPark, J. H., Schroeder, A. J., Helfrich-Förster, C., Jackson, F. R., & Ewer, J. (2003) · Development · doi:10.1242/dev.00503 · Cited by 224
Self-citationMorita, H., Mazerbourg, S., Bouley, D. M., Luo, C.-W., Kawamura, K., Kuwabara, Y., Baribault, H., Tian, H., & Hsueh, A. J. W. (2004) · Molecular and Cellular Biology · doi:10.1128/mcb.24.22.9736-9743.2004 · Cited by 187
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de Lau, W., Peng, W. C., Gros, P., & Clevers, H. (2014) · Genes & Development · doi:10.1101/gad.235473.113 · Cited by 656
Luan, H., Peabody, N. C., Vinson, C. R., & White, B. H. (2006) · Neuron · doi:10.1016/j.neuron.2006.08.028 · Cited by 500
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Pener, M. P., & Simpson, S. J. (2009) · Advances in Insect Physiology · doi:10.1016/s0065-2806(08)36001-9 · Cited by 481
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Metadata
- Authors
- Luo, C.-W., Dewey, E. M., Sudo, S., Ewer, J., Hsu, S. Y., Honegger, H.-W., & Hsueh, A. J. W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2005-02-09
- Volume
- 102
- Issue
- 8
- Pages
- 2820-2825
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1979276944
- Cited by
- 273
- References
- 20
- FWCI
- 6.11
- Type
- journal article
- Language
- en
- Open access
- green
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- Luo, C.-W., Dewey, E. M., Sudo, S., Ewer, J., Hsu, S. Y., Honegger, H.-W., & Hsueh, A. J. W. (2005). Bursicon, the insect cuticle-hardening hormone, is a heterodimeric cystine knot protein that activates G protein-coupled receptor LGR2. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0409916102
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