The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Predominant role of endothelial nitric oxide synthase in vascular endothelial growth factor-induced angiogenesis and vascular permeability
Open accessAbstract
Nitric oxide (NO) plays a critical role in vascular endothelial growth factor (VEGF)-induced angiogenesis and vascular hyperpermeability. However, the relative contribution of different NO synthase (NOS) isoforms to these processes is not known. Here, we evaluated the relative contributions of endothelial and inducible NOS (eNOS and iNOS, respectively) to angiogenesis and permeability of VEGF-induced angiogenic vessels. The contribution of eNOS was assessed by using an eNOS- deficient mouse, and iNOS contribution was assessed by using a selective inhibitor [ l - N 6 -(1-iminoethyl) lysine, l -NIL] and an iNOS- deficient mouse. Angiogenesis was induced by VEGF in type I collagen gels placed in the mouse cranial window. Angiogenesis, vessel diameter, blood flow rate, and vascular permeability were proportional to NO levels measured with microelectrodes: Wild-type (WT) ≥ WT with l -NIL or iNOS −/− > eNOS −/− ≥ eNOS −/− with l -NIL. The role of NOS in VEGF-induced acute vascular permeability increase in quiescent vessels also was determined by using eNOS- and iNOS- deficient mice. VEGF superfusion significantly increased permeability in both WT and iNOS −/− mice but not in eNOS −/− mice. These findings suggest that eNOS plays a predominant role in VEGF-induced angiogenesis and vascular permeability. Thus, selective modulation of eNOS activity is a promising strategy for altering angiogenesis and vascular permeability in vivo .
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Cites
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VEGF receptor signalling ? in control of vascular function
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Normalization of the Vasculature for Treatment of Cancer and Other Diseases
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Age-related macular degeneration: etiology, pathogenesis, and therapeutic strategies
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Metadata
- Authors
- Fukumura, D., Gohongi, T., Kadambi, A., Izumi, Y., Ang, J., Yun, C.-O., Buerk, D. G., Huang, P. L., & Jain, R. K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2001-02-27
- Volume
- 98
- Issue
- 5
- Pages
- 2604-2609
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1968719918
- Cited by
- 1,020
- References
- 45
- FWCI
- 12.27
- Type
- journal article
- Language
- en
- Open access
- green
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- Fukumura, D., Gohongi, T., Kadambi, A., Izumi, Y., Ang, J., Yun, C.-O., Buerk, D. G., Huang, P. L., & Jain, R. K. (2001). Predominant role of endothelial nitric oxide synthase in vascular endothelial growth factor-induced angiogenesis and vascular permeability. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.041359198
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
