The DOI matches its Crossref record.
Biological imaging by soft x-ray diffraction microscopy
Open accessAbstract
We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by using 750-eV x-rays from different angular orientations, portray several of the cell's major internal components to 30-nm resolution. The good agreement among the independently recovered structures demonstrates the accuracy of the imaging technique. To obtain the best possible reconstructions, we have implemented procedures for handling noisy and incomplete diffraction data, and we propose a method for determining the reconstructed resolution. This work represents a previously uncharacterized application of x-ray diffraction microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Single mimivirus particles intercepted and imaged with an X-ray laser
Seibert, M. M., Ekeberg, T., Maia, F. R. N. C., Svenda, M., Andreasson, J., Jönsson, O., Odić, D., Iwan, B., Rocker, A., Westphal, D., et al. (2011) · Nature · doi:10.1038/nature09748 · Cited by 904
Hard-X-Ray Lensless Imaging of Extended Objects
Rodenburg, J. M., Hurst, A. C., Cullis, A. G., Dobson, B. R., Pfeiffer, F., Bunk, O., David, C., Jefimovs, K., & Johnson, I. (2007) · Physical Review Letters · doi:10.1103/physrevlett.98.034801 · Cited by 855
Three-Dimensional Reconstruction of the Giant Mimivirus Particle with an X-Ray Free-Electron Laser
Ekeberg, T., Svenda, M., Abergel, C., Maia, F. R. N. C., Seltzer, V., Claverie, J.-M., Hantke, M., Jönsson, O., Nettelblad, C., van der Schot, G., et al. (2015) · Physical Review Letters · doi:10.1103/physrevlett.114.098102 · Cited by 344
Three-Dimensional Visualization of a Human Chromosome Using Coherent X-Ray Diffraction
Nishino, Y., Takahashi, Y., Imamoto, N., Ishikawa, T., & Maeshima, K. (2009) · Physical Review Letters · doi:10.1103/physrevlett.102.018101 · Cited by 306
Nuclear microscopy: A tool for imaging elemental distribution and percutaneous absorption in vivo
Veríssimo, A., Alves, L. C., Filipe, P., Silva, J. N., Silva, R., Dolores YNSA, M., Gontier, E., Moretto, P., Pallon, J., & Pinheiro, T. (2007) · Microscopy Research and Technique · doi:10.1002/jemt.20402 · Cited by 42
Metadata
- Authors
- Shapiro, D., Thibault, P., Beetz, T., Elser, V., Howells, M., Jacobsen, C., Kirz, J., Lima, E., Miao, H., Neiman, A. M., & Sayre, D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2005-10-11
- Volume
- 102
- Issue
- 43
- Pages
- 15343-15346
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2114520361
- Cited by
- 554
- References
- 0
- FWCI
- 30.47
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Biological imaging by soft x-ray diffraction microscopy. https://app.cochat.ai/papers/10.1073/pnas.0503305102
- The paper
- Shapiro, D., Thibault, P., Beetz, T., Elser, V., Howells, M., Jacobsen, C., Kirz, J., Lima, E., Miao, H., Neiman, A. M., & Sayre, D. (2005). Biological imaging by soft x-ray diffraction microscopy. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0503305102
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
