The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
The antiinflammatory effect of laminar flow: The role of PPARγ, epoxyeicosatrienoic acids, and soluble epoxide hydrolase
Open accessAbstract
We previously reported that laminar flow activates peroxisome proliferator-activated receptor γ (PPARγ) in vascular endothelial cells in a ligand-dependent manner that involves phospholipase A2 and cytochrome P450 epoxygenases. In this study, we investigated whether epoxyeicosatrienoic acids (EETs), the catalytic products of cytochrome P450 epoxygenases, are PPARγ ligands. Competition and direct binding assays revealed that EETs bind to the ligand-binding domain of PPARγ with K d in the μM range. In the presence of adamantyl-ureido-dodecanoic acid (AUDA), a soluble epoxide hydrolase (sEH)-specific inhibitor, EETs increased PPARγ transcription activity in endothelial cells and 3T3-L1 preadipocytes. Inclusion of AUDA in the perfusing media enhanced, but overexpression of sEH reduced, the laminar flow-induced PPARγ activity. Furthermore, laminar flow augmented cellular levels of EETs but decreased sEH at the levels of mRNA, protein, and activity. Blocking PPARγ by GW9662 abolished the EET/AUDA-mediated antiinflammatory effect, which indicates that PPARγ is an effector of EETs.
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15-Deoxy-Δ12,14-Prostaglandin J2 is a ligand for the adipocyte determination factor PPARγ
Forman, B. M., Tontonoz, P., Chen, J., Brun, R. P., Spiegelman, B. M., & Evans, R. M. (1995) · Cell · doi:10.1016/0092-8674(95)90193-0 · Cited by 2,858
Flow-mediated endothelial mechanotransduction
Davies, P. F. (1995) · Physiological Reviews · doi:10.1152/physrev.1995.75.3.519 · Cited by 2,802
Flow Effects on Prostacyclin Production by Cultured Human Endothelial Cells
Frangos, J. A., Eskin, S. G., McIntire, L. V., & Ives, C. L. (1985) · Science · doi:10.1126/science.3883488 · Cited by 1,235
Krey, G. D., Braissant, O., L’Horset, F., Kalkhoven, E., Perroud, M., Parker, M. G., & Wahli, W. (1997) · Molecular Endocrinology · doi:10.1210/me.11.6.779 · Cited by 1,221
Identification of Epoxyeicosatrienoic Acids as Endothelium-Derived Hyperpolarizing Factors
Campbell, W. B., Gebremedhin, D., Pratt, P. F., & Harder, D. R. (1996) · Circulation Research · doi:10.1161/01.res.78.3.415 · Cited by 1,221
Anti-inflammatory Properties of Cytochrome P450 Epoxygenase-Derived Eicosanoids
Node, K., Huo, Y., Ruan, X., Yang, B., Spiecker, M., Ley, K., Zeldin, D. C., & Liao, J. K. (1999) · Science · doi:10.1126/science.285.5431.1276 · Cited by 1,215
Iiyama, K., Hajra, L., Iiyama, M., Li, H., DiChiara, M., Medoff, B. D., & Cybulsky, M. I. (1999) · Circulation Research · doi:10.1161/01.res.85.2.199 · Cited by 632
Hajra, L., Evans, A. I., Chen, M., Hyduk, S. J., Collins, T., & Cybulsky, M. I. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.16.9052 · Cited by 574
Pasceri, V., Wu, H. D., Willerson, J. T., & Yeh, E. T. H. (2000) · Circulation · doi:10.1161/01.cir.101.3.235 · Cited by 504
Soluble Epoxide Hydrolase Inhibition Lowers Arterial Blood Pressure in Angiotensin II Hypertension
Imig, J. D., Zhao, X., Capdevila, J. H., Morisseau, C., & Hammock, B. D. (2002) · Hypertension · doi:10.1161/hy0202.103788 · Cited by 400
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Cited by
Eicosanoid storm in infection and inflammation
Dennis, E. A., & Norris, P. C. (2015) · Nature Reviews Immunology · doi:10.1038/nri3859 · Cited by 1,517
Impact of Soluble Epoxide Hydrolase and Epoxyeicosanoids on Human Health
Morisseau, C., & Hammock, B. D. (2013) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev-pharmtox-011112-140244 · Cited by 537
Self-citationAction of epoxyeicosatrienoic acids on cellular function
Spector, A. A., & Norris, A. W. (2007) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00402.2006 · Cited by 477
Self-citationEpoxides and Soluble Epoxide Hydrolase in Cardiovascular Physiology
Imig, J. D. (2012) · Physiological Reviews · doi:10.1152/physrev.00021.2011 · Cited by 365
Old and new generation lipid mediators in acute inflammation and resolution
Stables, M. J., & Gilroy, D. W. (2011) · Progress in Lipid Research · doi:10.1016/j.plipres.2010.07.005 · Cited by 334
Metadata
- Authors
- Liu, Y., Zhang, Y., Schmelzer, K., Lee, T.-S., Fang, X., Zhu, Y., Spector, A. A., Gill, S., Morisseau, C., Hammock, B. D., & Shyy, J. Y.-J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2005-11-02
- Volume
- 102
- Issue
- 46
- Pages
- 16747-16752
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2146083192
- Cited by
- 300
- References
- 27
- FWCI
- 5.20
- Type
- journal article
- Language
- en
- Open access
- green
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- Liu, Y., Zhang, Y., Schmelzer, K., Lee, T.-S., Fang, X., Zhu, Y., Spector, A. A., Gill, S., Morisseau, C., Hammock, B. D., & Shyy, J. Y.-J. (2005). The antiinflammatory effect of laminar flow: The role of PPARγ, epoxyeicosatrienoic acids, and soluble epoxide hydrolase. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0508081102
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