The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
A nucleoporin is required for induction of Ca 2+ spiking in legume nodule development and essential for rhizobial and fungal symbiosis
Open accessAbstract
Nuclear-cytoplasmic partitioning and traffic between cytoplasmic and nuclear compartments are fundamental processes in eukaryotic cells. Nuclear pore complexes mediate transport of proteins, RNAs and ribonucleoprotein particles in and out of the nucleus. Here we present positional cloning of a plant nucleoporin gene, Nup133 , essential for a symbiotic signal transduction pathway shared by Rhizobium bacteria and mycorrhizal fungi. Mutation of Nup133 results in a temperature sensitive nodulation deficient phenotype and absence of mycorrhizal colonization. Root nodules developing with reduced frequency at permissive temperatures are ineffective and electron microscopy show that Rhizobium bacteria are not released from infection threads. Measurement of ion fluxes using a calcium-sensitive dye show that Nup133 is required for the Ca 2+ spiking normally detectable within minutes after application of purified rhizobial Nod-factor signal molecules to root hairs. Localization of NUP133 in the nuclear envelope of root cells and root hair cells shown with enhanced yellow fluorescent protein fusion proteins suggests a novel role for NUP133 nucleoporins in a rapid nuclear–cytoplasmic communication after host–plant recognition of symbiotic microbes. Our results identify a component of an intriguing signal process requiring interaction at the cell plasma membrane and at intracellular nuclear and plastid organelle-membranes to induce a second messenger.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Innate immunity in plants and animals: striking similarities and obvious differences
Nürnberger, T., Brunner, F., Kemmerling, B., & Piater, L. (2004) · Immunological Reviews · doi:10.1111/j.0105-2896.2004.0119.x · Cited by 1,286
Plant recognition of symbiotic bacteria requires two LysM receptor-like kinases
Radutoiu, S., Madsen, L. H., Madsen, E. B., Felle, H. H., Umehara, Y., Grønlund, M., Sato, S., Nakamura, Y., Tabata, S., Sandal, N., et al. (2003) · Nature · doi:10.1038/nature02039 · Cited by 1,221
Self-citationA receptor kinase gene of the LysM type is involved in legumeperception of rhizobial signals
Madsen, E. B., Madsen, L. H., Radutoiu, S., Olbryt, M., Rakwalska, M., Szczyglowski, K., Sato, S., Kaneko, T., Tabata, S., Sandal, N., et al. (2003) · Nature · doi:10.1038/nature02045 · Cited by 1,018
Self-citationA plant receptor-like kinase required for both bacterial and fungal symbiosis
Stracke, S., Kistner, C., Yoshida, S., Mulder, L., Sato, S., Kaneko, T., Tabata, S., Sandal, N., Stougaard, J., Szczyglowski, K., et al. (2002) · Nature · doi:10.1038/nature00841 · Cited by 951
Self-citationA receptor kinase gene regulating symbiotic nodule development
Endre, G., Kereszt, A., Kevei, Z., Mihacea, S., Kaló, P., & Kiss, G. B. (2002) · Nature · doi:10.1038/nature00842 · Cited by 832
A Putative Ca 2+ and Calmodulin-Dependent Protein Kinase Required for Bacterial and Fungal Symbioses
Lévy, J., Bres, C., Geurts, R., Chalhoub, B., Kulikova, O., Duc, G., Journet, E.-P., Ané, J.-M., Lauber, E., Bisseling, T., et al. (2004) · Science · doi:10.1126/science.1093038 · Cited by 785
Calcium Spiking in Plant Root Hairs Responding to Rhizobium Nodulation Signals
Ehrhardt, D. W., Wais, R., & Long, S. R. (1996) · Cell · doi:10.1016/s0092-8674(00)81234-9 · Cited by 641
Medicago truncatula DMI1 Required for Bacterial and Fungal Symbioses in Legumes
Ané, J.-M., Kiss, G. B., Riely, B. K., Penmetsa, R. V., Oldroyd, G. E. D., Ayax, C., Lévy, J., Debellé, F., Baek, J.-M., Kalo, P., et al. (2004) · Science · doi:10.1126/science.1092986 · Cited by 552
MOLECULAR AND CELLULAR ASPECTS OF THE ARBUSCULAR MYCORRHIZAL SYMBIOSIS
Harrison, M. J. (1999) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.50.1.361 · Cited by 461
Plastid proteins crucial for symbiotic fungal and bacterial entry into plant roots
Imaizumi-Anraku, H., Takeda, N., Charpentier, M., Perry, J., Miwa, H., Umehara, Y., Kouchi, H., Murakami, Y., Mulder, L., Vickers, K., et al. (2005) · Nature · doi:10.1038/nature03237 · Cited by 416
Self-citation
Cited by
Hardoim, P. R., van Overbeek, L. S., Berg, G., Pirttilä, A. M., Compant, S., Campisano, A., Döring, M., & Sessitsch, A. (2015) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00050-14 · Cited by 2,708
How rhizobial symbionts invade plants: the Sinorhizobium–Medicago model
Jones, K. M., Kobayashi, H., Davies, B. W., Taga, M. E., & Walker, G. C. (2007) · Nature Reviews Microbiology · doi:10.1038/nrmicro1705 · Cited by 937
Invasive Plant Suppresses the Growth of Native Tree Seedlings by Disrupting Belowground Mutualisms
Stinson, K. A., Campbell, S. A., Powell, J. R., Wolfe, B. E., Callaway, R. M., Thelen, G. C., Hallett, S. G., Prati, D., & Klironomos, J. N. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040140 · Cited by 826
CYCLOPS, a mediator of symbiotic intracellular accommodation
Yano, K., Yoshida, S., Müller, J., Singh, S., Banba, M., Vickers, K., Markmann, K., White, C., Schuller, B., Sato, S., et al. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0806858105 · Cited by 532
Self-citationMarsh, J. F., Rakocevic, A., Mitra, R. M., Brocard, L., Sun, J., Eschstruth, A., Long, S. R., Schultze, M., Ratet, P., & Oldroyd, G. E. D. (2007) · Plant Physiology · doi:10.1104/pp.106.093021 · Cited by 462
Metadata
- Authors
- Kanamori, N., Madsen, L. H., Radutoiu, S., Frantescu, M., Quistgaard, E. M. H., Miwa, H., Downie, J. A., James, E. K., Felle, H. H., Haaning, L. L., Jensen, T. H., Sato, S., Nakamura, Y., Tabata, S., Sandal, N., & Stougaard, J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-01-03
- Volume
- 103
- Issue
- 2
- Pages
- 359-364
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2159753288
- Cited by
- 396
- References
- 31
- FWCI
- 39.89
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A nucleoporin is required for induction of Ca 2+ spiking in legume nodule development and essential for rhizobial and fungal symbiosis. https://app.cochat.ai/papers/10.1073/pnas.0508883103
- The paper
- Kanamori, N., Madsen, L. H., Radutoiu, S., Frantescu, M., Quistgaard, E. M. H., Miwa, H., Downie, J. A., James, E. K., Felle, H. H., Haaning, L. L., Jensen, T. H., Sato, S., Nakamura, Y., Tabata, S., Sandal, N., & Stougaard, J. (2006). A nucleoporin is required for induction of Ca 2+ spiking in legume nodule development and essential for rhizobial and fungal symbiosis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0508883103
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
