The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
CYCLOPS, a mediator of symbiotic intracellular accommodation
Open accessAbstract
The initiation of intracellular infection of legume roots by symbiotic rhizobia bacteria and arbuscular mycorrhiza (AM) fungi is preceded by the induction of calcium signatures in and around the nucleus of root epidermal cells. Although a calcium and calmodulin-dependent kinase (CCaMK) is a key mediator of symbiotic root responses, the decoding of the calcium signal and the molecular events downstream are only poorly understood. Here, we characterize Lotus japonicus cyclops mutants on which microbial infection was severely inhibited. In contrast, nodule organogenesis was initiated in response to rhizobia, but arrested prematurely. This arrest was overcome when a deregulated CCaMK mutant version was introduced into cyclops mutants, conferring the development of full-sized, spontaneous nodules. Because cyclops mutants block symbiotic infection but are competent for nodule development, they reveal a bifurcation of signal transduction downstream of CCaMK. We identified CYCLOPS by positional cloning. CYCLOPS carries a functional nuclear localization signal and a predicted coiled-coil domain. We observed colocalization and physical interaction between CCaMK and CYCLOPS in plant and yeast cell nuclei in the absence of symbiotic stimulation. Importantly, CYCLOPS is a phosphorylation substrate of CCaMK in vitro. Cyclops mutants of rice were impaired in AM, and rice CYCLOPS could restore symbiosis in Lotus cyclops mutants, indicating a functional conservation across angiosperms. Our results suggest that CYCLOPS forms an ancient, preassembled signal transduction complex with CCaMK that is specifically required for infection, whereas organogenesis likely requires additional yet-to-be identified CCaMK interactors or substrates.
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McGONIGLE, T. P., MILLER, M. H., EVANS, D. G., FAIRCHILD, G. L., & SWAN, J. A. (1990) · New Phytologist · doi:10.1111/j.1469-8137.1990.tb00476.x · Cited by 3,878
Arbuscular mycorrhiza: the mother of plant root endosymbioses
Parniske, M. (2008) · Nature Reviews Microbiology · doi:10.1038/nrmicro1987 · Cited by 2,167
Self-citationWalter, M., Chaban, C., Schütze, K., Batistic, O., Weckermann, K., Näke, C., Blazevic, D., Grefen, C., Schumacher, K., Oecking, C., et al. (2004) · The Plant Journal · doi:10.1111/j.1365-313x.2004.02219.x · Cited by 1,728
Hu, C.-D., Chinenov, Y., & Kerppola, T. K. (2002) · Molecular Cell · doi:10.1016/s1097-2765(02)00496-3 · Cited by 1,549
Plant recognition of symbiotic bacteria requires two LysM receptor-like kinases
Radutoiu, S., Madsen, L. H., Madsen, E. B., Felle, H. H., Umehara, Y., Grønlund, M., Sato, S., Nakamura, Y., Tabata, S., Sandal, N., et al. (2003) · Nature · doi:10.1038/nature02039 · Cited by 1,221
Self-citationFour hundred-million-year-old vesicular arbuscular mycorrhizae.
Remy, W., Taylor, T. N., Hass, H., & Kerp, H. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.25.11841 · Cited by 1,202
Coordinating Nodule Morphogenesis with Rhizobial Infection in Legumes
Oldroyd, G. E. D., & Downie, J. A. (2008) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.59.032607.092839 · Cited by 1,142
A plant receptor-like kinase required for both bacterial and fungal symbiosis
Stracke, S., Kistner, C., Yoshida, S., Mulder, L., Sato, S., Kaneko, T., Tabata, S., Sandal, N., Stougaard, J., Szczyglowski, K., et al. (2002) · Nature · doi:10.1038/nature00841 · Cited by 951
Self-citationA plant regulator controlling development of symbiotic root nodules
Schauser, L., Roussis, A., Stiller, J., & Stougaard, J. (1999) · Nature · doi:10.1038/46058 · Cited by 945
A receptor kinase gene regulating symbiotic nodule development
Endre, G., Kereszt, A., Kevei, Z., Mihacea, S., Kaló, P., & Kiss, G. B. (2002) · Nature · doi:10.1038/nature00842 · Cited by 832
Cited by
A phosphate starvation response-centered network regulates mycorrhizal symbiosis
Shi, J., Zhao, B., Zheng, S., Zhang, X., Wang, X., Dong, W., Xie, Q., Wang, G., Xiao, Y., Chen, F., et al. (2021) · Cell · doi:10.1016/j.cell.2021.09.030 · Cited by 384
Ladha, J. K., Peoples, M. B., Reddy, P. M., Biswas, J. C., Bennett, A., Jat, M. L., & Krupnik, T. J. (2022) · Field Crops Research · doi:10.1016/j.fcr.2022.108541 · Cited by 328
Invasion by Invitation: Rhizobial Infection in Legumes
Murray, J. D. (2011) · Molecular Plant-Microbe Interactions® · doi:10.1094/mpmi-08-10-0181 · Cited by 245
Roberts, N. J., Morieri, G., Kalsi, G., Rose, A., Stiller, J., Edwards, A., Xie, F., Gresshoff, P. M., Oldroyd, G. E. D., Downie, J. A., et al. (2012) · Plant Physiology · doi:10.1104/pp.112.206110 · Cited by 49
Metadata
- Authors
- Yano, K., Yoshida, S., Müller, J., Singh, S., Banba, M., Vickers, K., Markmann, K., White, C., Schuller, B., Sato, S., Asamizu, E., Tabata, S., Murooka, Y., Perry, J., Wang, T. L., Kawaguchi, M., Imaizumi-Anraku, H., Hayashi, M., & Parniske, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2008-12-23
- Volume
- 105
- Issue
- 51
- Pages
- 20540-20545
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2172019783
- Cited by
- 532
- References
- 46
- FWCI
- 23.91
- Type
- journal article
- Language
- en
- Open access
- green
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- Yano, K., Yoshida, S., Müller, J., Singh, S., Banba, M., Vickers, K., Markmann, K., White, C., Schuller, B., Sato, S., Asamizu, E., Tabata, S., Murooka, Y., Perry, J., Wang, T. L., Kawaguchi, M., Imaizumi-Anraku, H., Hayashi, M., & Parniske, M. (2008). CYCLOPS, a mediator of symbiotic intracellular accommodation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0806858105
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