The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Gold nanoparticles-based protease assay
Open accessAbstract
We describe here a simple assay that allows the visual detection of a protease. The method takes advantage of the high molar absorptivity of the plasmon band of gold colloids and is based on the color change of their solution when treated with dithiols. We used C- and N-terminal cysteinyl derivatives of a peptide substrate exploiting its selective recognition and cleavage by a specific protease. Contrary to the native ones, cleaved peptides are unable to induce nanoparticles aggregation; hence, the color of the solution does not change. The detection of two proteases is reported: thrombin (involved in blood coagulation and thrombosis) and lethal factor (an enzyme component of the toxin produced by Bacillus anthracis ). The sensitivity of this nanoparticle-based assay is in the low nanomolar range.
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Citations
Cites
Nanostructures in Biodiagnostics
Rosi, N. L., & Mirkin, C. A. (2005) · Chemical Reviews · doi:10.1021/cr030067f · Cited by 4,603
Cited by
Applications of Nanoparticles in Biology
De, M., Ghosh, P. S., & Rotello, V. M. (2008) · Advanced Materials · doi:10.1002/adma.200703183 · Cited by 1,547
Metadata
- Authors
- Guarise, C., Pasquato, L., De Filippis, V., & Scrimin, P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-03
- Volume
- 103
- Issue
- 11
- Pages
- 3978-3982
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2163523761
- Cited by
- 284
- References
- 1
- FWCI
- 13.52
- Type
- journal article
- Language
- en
- Open access
- green
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- Guarise, C., Pasquato, L., De Filippis, V., & Scrimin, P. (2006). Gold nanoparticles-based protease assay. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0509372103
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