The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Analysis of endoplasmic reticulum trafficking signals by combinatorial screening in mammalian cells
Open accessAbstract
To improve the accuracy of predicting membrane protein sorting signals, we developed a general methodology for defining trafficking signal consensus sequences in the environment of the living cell. Our approach uses retroviral gene transfer to create combinatorial expression libraries of trafficking signal variants in mammalian cells, flow cytometry to sort cells based on trafficking phenotype, and quantitative trafficking assays to measure the efficacy of individual signals. Using this strategy to analyze arginine- and lysine-based endoplasmic reticulum localization signals, we demonstrate that small changes in the local sequence context dramatically alter signal strength, generating a broad spectrum of trafficking phenotypes. Finally, using sequences from our screen, we found that the potency of di-lysine, but not di-arginine, mediated endoplasmic reticulum localization was correlated with the strength of interaction with α-COP.
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Cites
A Switch Between Two-, Three-, and Four-stranded Coiled Coils in GCN4 Leucine Zipper Mutants
Harbury, P. B., Zhang, T., Kim, P. S., & Alber, T. (1993) · Science · doi:10.1126/science.8248779 · Cited by 1,490
Cited by
Michelsen, K., Yuan, H., & Schwappach, B. (2005) · The EMBO Reports · doi:10.1038/sj.embor.7400480 · Cited by 235
Self-citationScott, D. B., Blanpied, T. A., & Ehlers, M. D. (2003) · Neuropharmacology · doi:10.1016/s0028-3908(03)00250-8 · Cited by 186
Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals
Schueren, F., Lingner, T., George, R., Hofhuis, J., Dickel, C., Gärtner, J., & Thoms, S. (2014) · eLife · doi:10.7554/elife.03640 · Cited by 180
Brock, C., Boudier, L., Maurel, D., Blahos, J., & Pin, J.-P. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e05-05-0400 · Cited by 81
Gassmann, M., Haller, C., Stoll, Y., Aziz, S. A., Biermann, B., Mosbacher, J., Kaupmann, K., & Bettler, B. (2005) · Molecular Pharmacology · doi:10.1124/mol.104.010256 · Cited by 56
Metadata
- Authors
- Zerangue, N., Malan, M. J., Fried, S. R., Dazin, P. F., Jan, Y. N., Jan, L. Y., & Schwappach, B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2001-02-27
- Volume
- 98
- Issue
- 5
- Pages
- 2431-2436
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1975034569
- Cited by
- 174
- References
- 1
- FWCI
- 3.98
- Type
- journal article
- Language
- en
- Open access
- green
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- Zerangue, N., Malan, M. J., Fried, S. R., Dazin, P. F., Jan, Y. N., Jan, L. Y., & Schwappach, B. (2001). Analysis of endoplasmic reticulum trafficking signals by combinatorial screening in mammalian cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.051630198
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
