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Cited by 180RNA and protein synthesis mechanismsRedox biology and oxidative stressGenomics and Phylogenetic Studies

Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals

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Schueren, Fabian, Lingner, Thomas, George, Rosemol, Hofhuis, Julia, Dickel, Corinna, Gärtner, Jutta, Thoms, Sven·2014·eLife
DOI 10.7554/elife.03640PMC PMC4359377
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Abstract

Translational readthrough gives rise to low abundance proteins with C-terminal extensions beyond the stop codon. To identify functional translational readthrough, we estimated the readthrough propensity (RTP) of all stop codon contexts of the human genome by a new regression model in silico, identified a nucleotide consensus motif for high RTP by using this model, and analyzed all readthrough extensions in silico with a new predictor for peroxisomal targeting signal type 1 (PTS1). Lactate dehydrogenase B (LDHB) showed the highest combined RTP and PTS1 probability. Experimentally we show that at least 1.6% of the total cellular LDHB is targeted to the peroxisome by a conserved hidden PTS1. The readthrough-extended lactate dehydrogenase subunit LDHBx can also co-import LDHA, the other LDH subunit, into peroxisomes. Peroxisomal LDH is conserved in mammals and likely contributes to redox equivalent regeneration in peroxisomes.

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Cited by

Sorted by citations

Authors
Schueren, F., Lingner, T., George, R., Hofhuis, J., Dickel, C., Gärtner, J., & Thoms, S.
Affiliations
  • Universitätsmedizin Göttingen (DE)
  • University of Göttingen (DE)
Corresponding author
Thoms, Sven
ORCID
Venue
eLife
Publisher
eLife Sciences Publications, Ltd
Published
2014-09-23
Volume
3
Pages
e03640-e03640
ISSN
2050-084X (ISSN-L)
DOI
10.7554/elife.03640
PMC
PMC4359377
OpenAlex
W2155346577
Cited by
180
References
2
Citations by year
2026: 9 · 2025: 11 · 2024: 17 · 2023: 9 · 2022: 23 · 2021: 16 · 2020: 20 · 2019: 16 · 2018: 12 · 2017: 11 · 2016: 18 · 2015: 18
FWCI
5.91
Type
journal article
Language
en
Open access
Licence
Locations
  • eLife (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by)
  • GRO (submittedVersion, cc-by)
Funders
  • Georg-August-Universität Göttingen (Research Program, Faculty of Medicine,)
  • Deutsche Forschungsgemeinschaft (LI2050/1-1, GA354/7-1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords
translational readthrough, LDHB, LDHA, peroxisome targeting, peroxisome biogenesis
MeSH terms
Animals, Base Sequence, Cell Line, Computer Simulation, Humans, Isoenzymes, L-Lactate Dehydrogenase, Mammals, Molecular Sequence Data, Protein Biosynthesis, Genome, Conserved Sequence, Genes, Reporter, Codon, Terminator, Peroxisomes, Protein Transport, Protein Sorting Signals, Rats

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