The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Defined culture conditions of human embryonic stem cells
Abstract
Human embryonic stem cells (hESCs) are pluripotent cells that have the potential to differentiate into any tissue in the human body; therefore, they are a valuable resource for regenerative medicine, drug screening, and developmental studies. However, the clinical application of hESCs is hampered by the difficulties of eliminating animal products in the culture medium and/or the complexity of conditions required to support hESC growth. We have developed a simple medium [termed hESC Cocktail (HESCO)] containing basic fibroblast growth factor, Wnt3a, April (a proliferation-inducing ligand)/BAFF (B cell-activating factor belonging to TNF), albumin, cholesterol, insulin, and transferrin, which is sufficient for hESC self-renewal and proliferation. Cells grown in HESCO were maintained in an undifferentiated state as determined by using six different stem cell markers, and their genomic integrity was confirmed by karyotyping. Cells cultured in HESCO readily form embryoid bodies in tissue culture and teratomas in mice. In both cases, the cells differentiated into each of the three cell lineages, ectoderm, endoderm, and mesoderm, indicating that they maintained their pluripotency. The use of a minimal medium sufficient for hESC growth is expected to greatly facilitate clinical application and developmental studies of hESCs.
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Cites
Wnt proteins are lipid-modified and can act as stem cell growth factors
Willert, K., Brown, J. D., Danenberg, E., Duncan, A. W., Weissman, I. L., Reya, T., Yates, J. R., & Nusse, R. (2003) · Nature · doi:10.1038/nature01611 · Cited by 2,257
Basement membrane complexes with biological activity
Kleinman, H. K., McGarvey, M. L., Hassell, J. R., Star, V. L., Cannon, F. B., Laurie, G. W., & Martin, G. R. (1986) · Biochemistry · doi:10.1021/bi00350a005 · Cited by 1,502
Derivation of human embryonic stem cells in defined conditions
Ludwig, T. E., Levenstein, M. E., Jones, J. M., Berggren, W. T., Mitchen, E. R., Frane, J. L., Crandall, L. J., Daigh, C. A., Conard, K. R., Piekarczyk, M. S., et al. (2006) · Nature Biotechnology · doi:10.1038/nbt1177 · Cited by 1,114
Cited by
Miyazaki, T., Futaki, S., Suemori, H., Taniguchi, Y., Yamada, M., Kawasaki, M., Hayashi, M., Kumagai, H., Nakatsuji, N., Sekiguchi, K., et al. (2012) · Nature Communications · doi:10.1038/ncomms2231 · Cited by 368
Wnt/β-catenin/CBP signaling maintains long-term murine embryonic stem cell pluripotency
Miyabayashi, T., Teo, J.-L., Yamamoto, M., McMillan, M., Nguyen, C., & Kahn, M. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0701331104 · Cited by 310
Glycolytic Metabolism Plays a Functional Role in Regulating Human Pluripotent Stem Cell State
Gu, W., Gaeta, X., Sahakyan, A., Chan, A. B., Hong, C. S., Kim, R., Braas, D., Plath, K., Lowry, W. E., & Christofk, H. R. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.08.008 · Cited by 283
High-Throughput Discovery of Synthetic Surfaces That Support Proliferation of Pluripotent Cells
Derda, R., Musah, S., Orner, B. P., Klim, J. R., Li, L., & Kiessling, L. L. (2010) · Journal of the American Chemical Society · doi:10.1021/ja906089g · Cited by 144
Cardiomyocytes from human pluripotent stem cells in regenerative medicine and drug discovery
Braam, S. R., Passier, R., & Mummery, C. L. (2009) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2009.07.001 · Cited by 86
Metadata
- Authors
- Lu, J., Hou, R., Booth, C. J., Yang, S.-H., & Snyder, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-04-11
- Volume
- 103
- Issue
- 15
- Pages
- 5688-5693
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W4247033596
- Cited by
- 200
- References
- 3
- FWCI
- 7.37
- Type
- journal article
- Language
- en
- Open access
- closed
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- Lu, J., Hou, R., Booth, C. J., Yang, S.-H., & Snyder, M. (2006). Defined culture conditions of human embryonic stem cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0601383103
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
