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Cited by 285

The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.

A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes

Open access
Goldberg, Susanne M. D., Johnson, Justin, Busam, Dana, Feldblyum, Tamara, Ferriera, Steve, Friedman, Robert, Halpern, Aaron, Khouri, Hoda, Kravitz, Saul A., Lauro, Federico M., Li, Kelvin, Rogers, Yu-Hui, Strausberg, Robert, Sutton, Granger, Tallon, Luke, Thomas, Torsten, Venter, Eli, Frazier, Marvin, Venter, J. Craig·2006·Proceedings of the National Academy of Sciences

Abstract

Since its introduction a decade ago, whole-genome shotgun sequencing (WGS) has been the main approach for producing cost-effective and high-quality genome sequence data. Until now, the Sanger sequencing technology that has served as a platform for WGS has not been truly challenged by emerging technologies. The recent introduction of the pyrosequencing-based 454 sequencing platform (454 Life Sciences, Branford, CT) offers a very promising sequencing technology alternative for incorporation in WGS. In this study, we evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730 xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.

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Crossref · 10.1073/pnas.0604351103

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Metadata

Authors
Goldberg, S. M. D., Johnson, J., Busam, D., Feldblyum, T., Ferriera, S., Friedman, R., Halpern, A., Khouri, H., Kravitz, S. A., Lauro, F. M., Li, K., Rogers, Y.-H., Strausberg, R., Sutton, G., Tallon, L., Thomas, T., Venter, E., Frazier, M., & Venter, J. C.
Venue
Proceedings of the National Academy of Sciences
Published
2006-07-25
Volume
103
Issue
30
Pages
11240-11245
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2141324508
Cited by
285
References
7
FWCI
16.58
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes. https://app.cochat.ai/papers/10.1073/pnas.0604351103
The paper
Goldberg, S. M. D., Johnson, J., Busam, D., Feldblyum, T., Ferriera, S., Friedman, R., Halpern, A., Khouri, H., Kravitz, S. A., Lauro, F. M., Li, K., Rogers, Y.-H., Strausberg, R., Sutton, G., Tallon, L., Thomas, T., Venter, E., Frazier, M., & Venter, J. C. (2006). A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0604351103

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.