The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Identification of a protein complex that assembles lipopolysaccharide in the outer membrane of Escherichia coli
Open accessAbstract
The outer membrane of most Gram-negative bacteria is made up of LPS, and in nearly all bacteria that contain LPS it is essential for the life of the organism. The lipid portion of this molecule, lipid A, also known as endotoxin, is a potent activator of the innate immune response. More than 50 genes are required to synthesize LPS and assemble it at the cell surface. Enormous progress has been made in elucidating the structure and biosynthesis of LPS, but until recently the cellular components required for its transport from its site of synthesis in the inner membrane to its final cellular location at the cell surface remained elusive. Here we describe the identification of a protein complex that functions to assemble LPS at the surface of the cell. This complex contains two proteins: Imp, already identified as an essential outer-membrane protein implicated in LPS assembly; and another protein, RlpB, heretofore identified only as a rare lipoprotein. We show that RlpB is also essential for cell viability and that the Imp/RlpB complex is responsible for LPS reaching the outer surface of the outer membrane.
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Cites
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Self-citationRecombineering: a powerful new tool for mouse functional genomics
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Cited by
Biosynthesis and Export of Bacterial Lipopolysaccharides
Whitfield, C., & Trent, M. S. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035600 · Cited by 709
Peptidomimetic Antibiotics Target Outer-Membrane Biogenesis in Pseudomonas aeruginosa
Srinivas, N., Jetter, P., Ueberbacher, B. J., Werneburg, M., Zerbe, K., Steinmann, J., Van der Meijden, B., Bernardini, F., Lederer, A., Dias, R. L. A., et al. (2010) · Science · doi:10.1126/science.1182749 · Cited by 554
β-Barrel Membrane Protein Assembly by the Bam Complex
Hagan, C. L., Silhavy, T. J., & Kahne, D. (2011) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-061408-144611 · Cited by 317
Self-citationSklar, J. G., Wu, T., Gronenberg, L. S., Malinverni, J. C., Kahne, D., & Silhavy, T. J. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0701579104 · Cited by 296
Self-citationStructural basis for lipopolysaccharide insertion in the bacterial outer membrane
Qiao, S., Luo, Q., Zhao, Y., Zhang, X. C., & Huang, Y. (2014) · Nature · doi:10.1038/nature13484 · Cited by 273
Metadata
- Authors
- Wu, T., McCandlish, A. C., Gronenberg, L. S., Chng, S.-S., Silhavy, T. J., & Kahne, D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-08
- Volume
- 103
- Issue
- 31
- Pages
- 11754-11759
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1980427104
- Cited by
- 366
- References
- 34
- FWCI
- 9.90
- Type
- journal article
- Language
- en
- Open access
- green
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