The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Experimental Determination and System Level Analysis of Essential Genes in Escherichia coli MG1655
Open accessAbstract
Defining the gene products that play an essential role in an organism's functional repertoire is vital to understanding the system level organization of living cells. We used a genetic footprinting technique for a genome-wide assessment of genes required for robust aerobic growth of Escherichia coli in rich media. We identified 620 genes as essential and 3,126 genes as dispensable for growth under these conditions. Functional context analysis of these data allows individual functional assignments to be refined. Evolutionary context analysis demonstrates a significant tendency of essential E. coli genes to be preserved throughout the bacterial kingdom. Projection of these data over metabolic subsystems reveals topologic modules with essential and evolutionarily preserved enzymes with reduced capacity for error tolerance.
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Cites
Functional profiling of the Saccharomyces cerevisiae genome
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Hierarchical Organization of Modularity in Metabolic Networks
Ravasz, E., Somera, A. L., Mongru, D. A., Oltvai, Z. N., & Barabási, A.-L. (2002) · Science · doi:10.1126/science.1073374 · Cited by 4,224
Self-citationComputational systems biology.
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Reverse engineering of biological complexity
Csete, M. E., & Doyle, J. C. (2002) · Science · doi:10.1126/science.1069981 · Cited by 1,215
Metabolic network structure determines key aspects of functionality and regulation
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Metabolic modelling of microbes: the flux‐balance approach
Edwards, J. S., Covert, M., & Palsson, B. (2002) · Environmental Microbiology · doi:10.1046/j.1462-2920.2002.00282.x · Cited by 365
Cited by
Network biology: understanding the cell's functional organization
Barabási, A.-L., & Oltvai, Z. N. (2004) · Nature Reviews Genetics · doi:10.1038/nrg1272 · Cited by 7,903
Self-citationConstruction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection
Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M., Datsenko, K. A., Tomita, M., Wanner, B. L., & Mori, H. (2006) · Molecular Systems Biology · doi:10.1038/msb4100050 · Cited by 7,858
Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt520 · Cited by 1,145
Robustness of cellular functions
Stelling, J., Sauer, U., Szallasi, Z., Doyle, F. J., & Doyle, J. (2004) · Cell · doi:10.1016/j.cell.2004.09.008 · Cited by 1,049
Comparative genomics, minimal gene-sets and the last universal common ancestor
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Metadata
- Authors
- Gerdes, S. Y., Scholle, M. D., Campbell, J. W., Balázsi, G., Ravasz, E., Daugherty, M. D., Somera, A. L., Kyrpides, N. C., Anderson, I., Gelfand, M. S., Bhattacharya, A., Kapatral, V., D'Souza, M., Baev, M. V., Grechkin, Y., Mseeh, F., Fonstein, M. Y., Overbeek, R., Barabási, A.-L., Oltvai, Z. N., & Osterman, A. L.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2003-10
- Volume
- 185
- Issue
- 19
- Pages
- 5673-5684
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2146538795
- Cited by
- 725
- References
- 6
- FWCI
- 18.92
- Type
- journal article
- Language
- en
- Open access
- green
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- Gerdes, S. Y., Scholle, M. D., Campbell, J. W., Balázsi, G., Ravasz, E., Daugherty, M. D., Somera, A. L., Kyrpides, N. C., Anderson, I., Gelfand, M. S., Bhattacharya, A., Kapatral, V., D'Souza, M., Baev, M. V., Grechkin, Y., Mseeh, F., Fonstein, M. Y., Overbeek, R., Barabási, A.-L., . . . Osterman, A. L. (2003). Experimental Determination and System Level Analysis of Essential Genes in Escherichia coli MG1655. Journal of Bacteriology. https://doi.org/10.1128/jb.185.19.5673-5684.2003
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
