The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
FRET imaging in living maize cells reveals that plasma membrane aquaporins interact to regulate their subcellular localization
Open accessAbstract
Zea mays plasma membrane intrinsic proteins (ZmPIPs) fall into two groups, ZmPIP1s and ZmPIP2s, that exhibit different water channel activities when expressed in Xenopus oocytes. ZmPIP1s are inactive, whereas ZmPIP2s induce a marked increase in the membrane osmotic water permeability coefficient, P f . We previously showed that, in Xenopus oocytes, ZmPIP1;2 and ZmPIP2;1 interact to increase the cell P f . Here, we report the localization and interaction of ZmPIP1s and ZmPIP2s in living maize cells. ZmPIPs were fused to monomeric yellow fluorescent protein and/or monomeric cyan fluorescent protein and expressed transiently in maize mesophyll protoplasts. When expressed alone, ZmPIP1 fusion proteins were retained in the endoplasmic reticulum, whereas ZmPIP2s were found in the plasma membrane. Interestingly, when coexpressed with ZmPIP2s, ZmPIP1s were relocalized to the plasma membrane. Using FRET/fluorescence lifetime imaging microscopy, we demonstrated that this relocalization results from interaction between ZmPIP1s and ZmPIP2s. Immunoprecipitation experiments provided additional evidence for the association of ZmPIP1;2 and ZmPIP2;1 in maize roots and suspension cells. These data suggest that PIP1–PIP2 interaction is required for in planta PIP1 trafficking to the plasma membrane to modulate plasma membrane permeability.
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Cites
Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells
Zacharias, D. A., Violin, J. D., Newton, A. C., & Tsien, R. Y. (2002) · Science · doi:10.1126/science.1068539 · Cited by 2,235
Structural determinants of water permeation through aquaporin-1
Murata, K., Mitsuoka, K., Hirai, T., Walz, T., Agre, P., Heymann, J. B., Engel, A., & Fujiyoshi, Y. (2000) · Nature · doi:10.1038/35036519 · Cited by 1,812
Haseloff, J., Siemering, K. R., Prasher, D. C., & Hodge, S. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.6.2122 · Cited by 1,322
Structural basis of water-specific transport through the AQP1 water channel
Sui, H., Han, B.-G., Lee, J. K., Walian, P., & Jap, B. K. (2001) · Nature · doi:10.1038/414872a · Cited by 1,166
Structural mechanism of plant aquaporin gating
Törnroth-Horsefield, S., Wang, Y., Hedfalk, K., Johanson, U., Karlsson, M., Tajkhorshid, E., Neutze, R., & Kjellbom, P. (2006) · Nature · doi:10.1038/nature04316 · Cited by 862
Lipid–protein interactions in double-layered two-dimensional AQP0 crystals
Gonen, T., Cheng, Y., Sliz, P., Hiroaki, Y., Fujiyoshi, Y., Harrison, S. C., & Walz, T. (2005) · Nature · doi:10.1038/nature04321 · Cited by 662
Aquaporins Constitute a Large and Highly Divergent Protein Family in Maize
Chaumont, F., Barrieu, F., Wojcik, E., Chrispeels, M. J., & Jung, R. (2001) · Plant Physiology · doi:10.1104/pp.125.3.1206 · Cited by 644
Self-citationThe tobacco aquaporin NtAQP1 is a membrane CO2 pore with physiological functions
Uehlein, N., Lovisolo, C., Siefritz, F., & Kaldenhoff, R. (2003) · Nature · doi:10.1038/nature02027 · Cited by 635
Batoko, H., Zheng, H.-Q., Hawes, C., & Moore, I. (2000) · The Plant Cell · doi:10.1105/tpc.12.11.2201 · Cited by 562
Self-citationHeterodimerization and Endocytosis of Arabidopsis Brassinosteroid Receptors BRI1 and AtSERK3 (BAK1)
Russinova, E., Borst, J.-W., Kwaaitaal, M., Caño-Delgado, A., Yin, Y., Chory, J., & de Vries, S. C. (2004) · The Plant Cell · doi:10.1105/tpc.104.025387 · Cited by 478
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Maurel, C., Boursiac, Y., Luu, D.-T., Santoni, V., Shahzad, Z., & Verdoucq, L. (2015) · Physiological Reviews · doi:10.1152/physrev.00008.2015 · Cited by 940
Role of aquaporins in leaf physiology
Heinen, R. B., Ye, Q., & Chaumont, F. (2009) · Journal of Experimental Botany · doi:10.1093/jxb/erp171 · Cited by 304
Self-citationFluorescence lifetime imaging microscopy in life sciences
Borst, J. W., & Visser, A. J. W. G. (2010) · Measurement Science and Technology · doi:10.1088/0957-0233/21/10/102002 · Cited by 168
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Metadata
- Authors
- Zelazny, E., Borst, J. W., Muylaert, M., Batoko, H., Hemminga, M. A., & Chaumont, F.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2007-07-24
- Volume
- 104
- Issue
- 30
- Pages
- 12359-12364
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2072540327
- Cited by
- 323
- References
- 38
- FWCI
- 7.90
- Type
- journal article
- Language
- en
- Open access
- green
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- Zelazny, E., Borst, J. W., Muylaert, M., Batoko, H., Hemminga, M. A., & Chaumont, F. (2007). FRET imaging in living maize cells reveals that plasma membrane aquaporins interact to regulate their subcellular localization. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0701180104
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