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Cited by 280

The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.

The micro-Petri dish, a million-well growth chip for the culture and high-throughput screening of microorganisms

Open access
Ingham, Colin J., Sprenkels, Ad, Bomer, Johan, Molenaar, Douwe, van den Berg, Albert, van Hylckama Vlieg, Johan E. T., de Vos, Willem M.·2007·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.0701693104
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Abstract

A miniaturized, disposable microbial culture chip has been fabricated by microengineering a highly porous ceramic sheet with up to one million growth compartments. This versatile culture format, with discrete compartments as small as 7 × 7 μm, allowed the growth of segregated microbial samples at an unprecedented density. The chip has been used for four complementary applications in microbiology. ( i ) As a fast viable counting system that showed a dynamic range of over 10,000, a low degree of bias, and a high culturing efficiency. ( ii ) In high-throughput screening, with the recovery of 1 fluorescent microcolony in 10,000. ( iii ) In screening for an enzyme-based, nondominant phenotype by the targeted recovery of Escherichia coli transformed with the plasmid pUC18, based on expression of the lacZ reporter gene without antibiotic-resistance selection. The ease of rapid, successive changes in the environment of the organisms on the chip, needed for detection of β-galactosidase activity, highlights an advantageous feature that was also used to screen a metagenomic library for the same activity. ( iv ) In high-throughput screening of >200,000 isolates from Rhine water based on metabolism of a fluorogenic organophosphate compound, resulting in the recovery of 22 microcolonies with the desired phenotype. These isolates were predicted, on the basis of rRNA sequence, to include six new species. These four applications suggest that the potential for such simple, readily manufactured chips to impact microbial culture is extensive and may facilitate the full automation and multiplexing of microbial culturing, screening, counting, and selection.

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Metadata

Authors
Ingham, C. J., Sprenkels, A., Bomer, J., Molenaar, D., van den Berg, A., van Hylckama Vlieg, J. E. T., & de Vos, W. M.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
2007-11-13
Volume
104
Issue
46
Pages
18217-18222
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.0701693104
OpenAlex
W1992837433
Cited by
280
References
24
FWCI
16.00
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: The micro-Petri dish, a million-well growth chip for the culture and high-throughput screening of microorganisms. https://app.cochat.ai/papers/10.1073/pnas.0701693104
The paper
Ingham, C. J., Sprenkels, A., Bomer, J., Molenaar, D., van den Berg, A., van Hylckama Vlieg, J. E. T., & de Vos, W. M. (2007). The micro-Petri dish, a million-well growth chip for the culture and high-throughput screening of microorganisms. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0701693104

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.