The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Single-vesicle imaging reveals that synaptic vesicle exocytosis and endocytosis are coupled by a single stochastic mode
Open accessAbstract
The nature of synaptic vesicle recycling at nerve terminals has been a subject of considerable debate for >35 years. Here, we report the use of an optical strategy that allows the exocytosis and retrieval of synaptic components to be tracked in real time at single-molecule sensitivity in living nerve terminals. This approach has allowed us to examine the recycling of synaptic vesicles in response to single action potentials. Our results show that, after exocytosis, individual synaptic vesicles are retrieved by a stochastic process with an exponential distribution of delay times, with a mean time of approximately 14 s. We propose that evidence for fast endocytosis, such as that proposed to support the presence of kiss-and-run, is likely explained by the stochastic nature of a slower process.
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In the citing paper
“vGpH, which increases in green fluorescence intensity upon vesicle fusion12.”
From A trans-synaptic nanocolumn aligns neurotransmitter release to receptors (2016) · cites this paper as [12]
In the citing paper
“When ultrafast endocytosis is blocked, a slow clathrin-mediated endocytic pathway can kick in7 - this is consistent with observations from live-cell imaging of endocytosis68–70.”
From Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis (2023) · cites this paper as [68]
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Cites
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A Selective Activity-Dependent Requirement for Dynamin 1 in Synaptic Vesicle Endocytosis
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Self-citationDynamics of synaptic vesicle fusion and membrane retrieval in synaptic terminals
von Gersdorff, H., & Mathews, G. (1994) · Nature · doi:10.1038/367735a0 · Cited by 457
Three modes of synaptic vesicular recycling revealed by single-vesicle imaging
Gandhi, S., & Stevens, C. F. (2003) · Nature · doi:10.1038/nature01677 · Cited by 452
Distinct Endocytic Pathways Control the Rate and Extent of Synaptic Vesicle Protein Recycling
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Kinetics and regulation of fast endocytosis at hippocampal synapses
Klingauf, J., Kavalali, E. T., & Tsien, R. W. (1998) · Nature · doi:10.1038/29079 · Cited by 415
Cited by
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Sun, F., Zeng, J., Jing, M., Zhou, J., Feng, J., Owen, S. F., Luo, Y., Li, F., Wang, H., Yamaguchi, T., et al. (2018) · Cell · doi:10.1016/j.cell.2018.06.042 · Cited by 837
Activity-driven local ATP synthesis is required for synaptic function
Rangaraju, V., Calloway, N., & Ryan, T. A. (2014) · Cell · doi:10.1016/j.cell.2013.12.042 · Cited by 836
Self-citationA trans-synaptic nanocolumn aligns neurotransmitter release to receptors
Tang, A.-H., Chen, H., Li, T. P., Metzbower, S. R., MacGillavry, H. D., & Blanpied, T. A. (2016) · Nature · doi:10.1038/nature19058 · Cited by 677
Ultrafast endocytosis at mouse hippocampal synapses
Watanabe, S., Rost, B. R., Camacho-Pérez, M., Davis, M. W., Söhl-Kielczynski, B., Rosenmund, C., & Jorgensen, E. M. (2013) · Nature · doi:10.1038/nature12809 · Cited by 607
Saheki, Y., & De Camilli, P. (2012) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a005645 · Cited by 457
Metadata
- Authors
- Balaji, J., & Ryan, T. A.
- Affiliations
- Weill Cornell Medicine (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2007-12-18
- Volume
- 104
- Issue
- 51
- Pages
- 20576-20581
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC2154473
- OpenAlex
- W2008643711
- Cited by
- 279
- References
- 24
- Citations by year
- 2026: 5 · 2025: 12 · 2024: 11 · 2023: 7 · 2022: 18 · 2021: 12 · 2020: 14 · 2019: 17 · 2018: 18 · 2017: 19 · 2016: 12 · 2015: 14 · 2014: 23 · 2013: 20 · 2012: 10
- FWCI
- 7.01
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- University of California, San Francisco
- National Institute of Neurological Disorders and Stroke (R01NS036942)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- synaptic vesicle recycling, exocytosis, endocytosis, kiss-and-run, single-vesicle imaging, single-molecule tracking, presynaptic terminals, synaptic transmission
- MeSH terms
- Animals, Cells, Cultured, Endocytosis, Exocytosis, Microscopy, Fluorescence, Synaptic Vesicles, Rats, Sprague-Dawley, Photobleaching, Green Fluorescent Proteins, Rats
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