The DOI matches its Crossref record.
A self-recombining bacterial artificial chromosome and its application for analysis of herpesvirus pathogenesis
Open accessAbstract
A self-recombining bacterial artificial chromosome (BAC) containing the 142-kb pseudorabies virus genome was constructed such that the viral genome is rapidly excised from the BAC vector backbone on delivery into mammalian cells. The recombination is mediated by loxP sites in the plasmid and Cre recombinase encoded within the BAC vector. A synthetic intron inserted in the middle of the cre ORF completely inhibits recombination in Escherichia coli , but is spliced out after delivery of the plasmid into mammalian cells. Recombination is efficient, and pure virus lacking the BAC vector backbone is immediately isolated from transfected mammalian cells without the need of serial passage or plaque purification.
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Cited by
Molecular Biology of Pseudorabies Virus: Impact on Neurovirology and Veterinary Medicine
Pomeranz, L. E., Reynolds, A. E., & Hengartner, C. J. (2005) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.69.3.462-500.2005 · Cited by 869
Peptide-specific recognition of human cytomegalovirus strains controls adaptive natural killer cells
Hammer, Q., Rückert, T., Borst, E. M., Dunst, J., Haubner, A., Durek, P., Heinrich, F., Gasparoni, G., Babic, M., Tomic, A., et al. (2018) · Nature Immunology · doi:10.1038/s41590-018-0082-6 · Cited by 423
Herpesviruses use bidirectional fast-axonal transport to spread in sensory neurons
Smith, G. A., Gross, S. P., & Enquist, L. W. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.061029798 · Cited by 269
Self-citationStanton, R. J., Baluchova, K., Dargan, D. J., Cunningham, C., Sheehy, O., Seirafian, S., McSharry, B. P., Neale, M. L., Davies, J. A., Tomasec, P., et al. (2010) · Journal of Clinical Investigation · doi:10.1172/jci42955 · Cited by 256
Nectow, A. R., & Nestler, E. J. (2020) · Nature reviews. Neuroscience · doi:10.1038/s41583-020-00382-z · Cited by 159
Metadata
- Authors
- Smith, G. A., & Enquist, L. W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2000-04-18
- Volume
- 97
- Issue
- 9
- Pages
- 4873-4878
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2159292028
- Cited by
- 200
- References
- 0
- FWCI
- 9.79
- Type
- journal article
- Language
- en
- Open access
- green
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- Smith, G. A., & Enquist, L. W. (2000). A self-recombining bacterial artificial chromosome and its application for analysis of herpesvirus pathogenesis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.080502497
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
