The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Expanding metabolism for biosynthesis of nonnatural alcohols
Open accessAbstract
Nature uses a limited set of metabolites to perform all of the biochemical reactions. To increase the metabolic capabilities of biological systems, we have expanded the natural metabolic network, using a nonnatural metabolic engineering approach. The branched-chain amino acid pathways are extended to produce abiotic longer chain keto acids and alcohols by engineering the chain elongation activity of 2-isopropylmalate synthase and altering the substrate specificity of downstream enzymes through rational protein design. When introduced into Escherichia coli , this nonnatural biosynthetic pathway produces various long-chain alcohols with carbon number ranging from 5 to 8. In particular, we demonstrate the feasibility of this approach by optimizing the biosynthesis of the 6-carbon alcohol, ( S )-3-methyl-1-pentanol. This work demonstrates an approach to build artificial metabolism beyond the natural metabolic network. Nonnatural metabolites such as long chain alcohols are now included in the metabolite family of living systems.
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Cites
Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection
Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M., Datsenko, K. A., Tomita, M., Wanner, B. L., & Mori, H. (2006) · Molecular Systems Biology · doi:10.1038/msb4100050 · Cited by 7,858
Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
Atsumi, S., Hanai, T., & Liao, J. C. (2008) · Nature · doi:10.1038/nature06450 · Cited by 1,945
Self-citationDieterich, D. C., Link, A. J., Graumann, J., Tirrell, D. A., & Schuman, E. M. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0601637103 · Cited by 874
Cited by
Current challenges in commercially producing biofuels from lignocellulosic biomass
Balan, V. (2014) · ISRN Biotechnology · doi:10.1155/2014/463074 · Cited by 551
Transformation of Biomass into Commodity Chemicals Using Enzymes or Cells
Straathof, A. J. J. (2014) · Chemical Reviews · doi:10.1021/cr400309c · Cited by 459
Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering
Quintana, N., Van der Kooy, F., Van de Rhee, M. D., Voshol, G. P., & Verpoorte, R. (2011) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3394-0 · Cited by 338
Application of synthetic biology in cyanobacteria and algae
Wang, B., Wang, J., Zhang, W., & Meldrum, D. R. (2012) · Frontiers in Microbiology · doi:10.3389/fmicb.2012.00344 · Cited by 211
Advances and prospects in metabolic engineering of Zymomonas mobilis
Wang, X., He, Q., Yang, Y., Wang, J., Haning, K., Hu, Y., Wu, B., He, M., Zhang, Y., Bao, J., et al. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2018.04.001 · Cited by 154
Metadata
- Authors
- Zhang, K., Sawaya, M. R., Eisenberg, D. S., & Liao, J. C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2008-12-30
- Volume
- 105
- Issue
- 52
- Pages
- 20653-20658
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2169989540
- Cited by
- 412
- References
- 3
- FWCI
- 10.17
- Type
- journal article
- Language
- en
- Open access
- green
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- Zhang, K., Sawaya, M. R., Eisenberg, D. S., & Liao, J. C. (2008). Expanding metabolism for biosynthesis of nonnatural alcohols. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0807157106
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