Application of synthetic biology in cyanobacteria and algae
Open accessAbstract
Cyanobacteria and algae are becoming increasingly attractive cell factories for producing renewable biofuels and chemicals due to their ability to capture solar energy and CO(2) and their relatively simple genetic background for genetic manipulation. Increasing research efforts from the synthetic biology approach have been made in recent years to modify cyanobacteria and algae for various biotechnological applications. In this article, we critically review recent progresses in developing genetic tools for characterizing or manipulating cyanobacteria and algae, the applications of genetically modified strains for synthesizing renewable products such as biofuels and chemicals. In addition, the emergent challenges in the development and application of synthetic biology for cyanobacteria and algae are also discussed.
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Native promoters, often sourced from cyanobacterial genes related to photosynthesis, carbon metabolism, or stress responses, are frequently used due to their compatibility with the host's transcriptional machinery.
“Native promoters, often sourced from cyanobacterial genes related to photosynthesis, carbon metabolism, or stress responses, are frequently used due to their compatibility with the host’s transcriptional machinery [98].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [98]
The “Development of BioBricks” section says native cyanobacterial promoters used are usually from photosynthesis-related genes, including those involved in CO₂ fixation, and gives a nickel-inducible promoter as another example. It notes that foreign E. coli promoters may behave differently in cyanobacteria, but does not say native promoters are frequently used because of compatibility with host transcriptional machinery or identify stress-response genes as a usual source. (LLM, conf=0.84)
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Sengupta, A., Pakrasi, H. B., & Wangikar, P. P. (2018) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-018-9046-x · Cited by 78 · see citing works
Engineered Transcriptional Systems for Cyanobacterial Biotechnology
Camsund, D., & Lindblad, P. (2014) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2014.00040 · Cited by 70 · see citing works
Recent Progress and Challenges in CRISPR-Cas9 Engineered Algae and Cyanobacteria
Patel, V. K., Das, A., Kumari, R., & Kajla, S. (2023) · Algal Research · doi:10.1016/j.algal.2023.103068 · Cited by 50 · see citing works
Recent advances in synthetic biology of cyanobacteria for improved chemicals production
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Potential of Producing Flavonoids Using Cyanobacteria as a Sustainable Chassis
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The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories
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- Authors
- Wang, B., Wang, J., Zhang, W., & Meldrum, D. R.
- Affiliations
- Arizona State University (US)
- Corresponding author
- Wang, Bo
- Venue
- Frontiers in Microbiology
- Publisher
- Frontiers Media SA
- Published
- 2012
- Volume
- 3
- Pages
- 344-344
- ISSN
- 1664-302X (ISSN-L)
- PMC
- PMC3446811
- OpenAlex
- W2061453352
- Cited by
- 211
- References
- 213
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 12 · 2023: 9 · 2022: 10 · 2021: 19 · 2020: 16 · 2019: 19 · 2018: 17 · 2017: 17 · 2016: 15 · 2015: 24 · 2014: 25 · 2013: 15 · 2012: 1
- FWCI
- 8.36
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Frontiers in Microbiology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Key Research and Development Program of China (2011CBA00803)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Keywords
- synthetic biology, cyanobacteria, algae, genetic instruments, genetically modified microorganisms, biofuels, renewable chemicals, cell factories, CO2 fixation, solar energy conversion
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