The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell
Open accessAbstract
We demonstrate far-field optical imaging with subdiffraction resolution of the endoplasmic reticulum (ER) in the interior of a living mammalian cell. The diffraction barrier is overcome by applying stimulated emission depletion (STED) on a yellow fluorescent protein tag. Imaging individual structural elements of the ER revealed a focal plane ( x , y ) resolution of <50 nm inside the living cell, corresponding to a 4-fold improvement over that of a confocal microscope and a 16-fold reduction in the focal-spot cross-sectional area. A similar gain in resolution is realized with both pulsed- and continuous-wave laser illumination. Images of highly convoluted parts of the ER reveal a similar resolution improvement in 3D optical sectioning by a factor of 3 along the optic axis ( z ). Time-lapse STED recordings document morphological changes of the ER over time. Thus, nanoscale 3D imaging of organelles in the interior of living cells greatly expands the scope of light microscopy in cell biology.
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Hell, S. W. (2007) · Science · doi:10.1126/science.1137395 · Cited by 2,967
Self-citationHighly Nonlinear Photodamage in Two-Photon Fluorescence Microscopy
Hopt, A., & Neher, E. (2001) · Biophysical Journal · doi:10.1016/s0006-3495(01)76173-5 · Cited by 442
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Grotjohann, T., Testa, I., Leutenegger, M., Bock, H., Urban, N. T., Lavoie-Cardinal, F., Willig, K. I., Eggeling, C., Jakobs, S., & Hell, S. W. (2011) · Nature · doi:10.1038/nature10497 · Cited by 501
Self-citationLive-cell imaging of dendritic spines by STED microscopy
Nägerl, U. V., Willig, K. I., Hein, B., Hell, S. W., & Bonhoeffer, T. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0810028105 · Cited by 412
Self-citationStimulated Emission Depletion Microscopy
Blom, H., & Widengren, J. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00653 · Cited by 339
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Wang, J., & Richards, D. A. (2012) · Biology Open · doi:10.1242/bio.20122071 · Cited by 146
Metadata
- Authors
- Hein, B., Willig, K. I., & Hell, S. W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2008-09-23
- Volume
- 105
- Issue
- 38
- Pages
- 14271-14276
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2004032495
- Cited by
- 475
- References
- 2
- FWCI
- 16.09
- Type
- journal article
- Language
- en
- Open access
- green
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- Hein, B., Willig, K. I., & Hell, S. W. (2008). Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0807705105
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