The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Site-specific chemical modification of recombinant proteins produced in mammalian cells by using the genetically encoded aldehyde tag
Open accessAbstract
The properties of therapeutic proteins can be enhanced by chemical modification. Methods for site-specific protein conjugation are critical to such efforts. Here, we demonstrate that recombinant proteins expressed in mammalian cells can be site-specifically modified by using a genetically encoded aldehyde tag. We introduced the peptide sequence recognized by the endoplasmic reticulum (ER)-resident formylglycine generating enzyme (FGE), which can be as short as 6 residues, into heterologous proteins expressed in mammalian cells. Cotranslational modification of the proteins by FGE produced products bearing a unique aldehyde group. Proteins bearing this “aldehyde tag” were chemically modified by selective reaction with hydrazide- or aminooxy-functionalized reagents. We applied the technique to site-specific modification of monoclonal antibodies, the fastest growing class of biopharmaceuticals, as well as membrane-associated and cytosolic proteins expressed in mammalian cells.
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Effect of pegylation on pharmaceuticals
Harris, J. M., & Chess, R. B. (2003) · Nature Reviews Drug Discovery · doi:10.1038/nrd1033 · Cited by 3,431
Protein Therapeutics: A Summary and Pharmacological Classification
Leader, B., Baca, Q. J., & Golan, D. E. (2008) · Nature Reviews Drug Discovery · doi:10.1038/nrd2399 · Cited by 2,161
Anti-Inflammatory Activity of Immunoglobulin G Resulting from Fc Sialylation
Kaneko, Y., Nimmerjahn, F., & Ravetch, J. V. (2006) · Science · doi:10.1126/science.1129594 · Cited by 1,754
Recapitulation of IVIG anti-inflammatory activity with a recombinant IgG Fc
Anthony, R. M., Nimmerjahn, F., Ashline, D. J., Reinhold, V. N., Paulson, J. C., & Ravetch, J. V. (2008) · Science · doi:10.1126/science.1154315 · Cited by 849
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Sletten, E. M., & Bertozzi, C. R. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200900942 · Cited by 3,165
Self-citationSite‐Specific and Stoichiometric Modification of Antibodies by Bacterial Transglutaminase
Jeger, S., Zimmermann, K., Blanc, A., Grünberg, J., Honer, M., Hunziker, P., Struthers, H., & Schibli, R. (2010) · Angewandte Chemie International Edition · doi:10.1002/anie.201004243 · Cited by 344
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MacDonald, J. I., Munch, H. K., Moore, T., & Francis, M. B. (2015) · Nature Chemical Biology · doi:10.1038/nchembio.1792 · Cited by 317
Srikun, D., Albers, A. E., Nam, C. I., Iavarone, A. T., & Chang, C. J. (2010) · Journal of the American Chemical Society · doi:10.1021/ja100117u · Cited by 310
Sequencing abasic sites in DNA at single-nucleotide resolution
Liu, Z. J., Martínez Cuesta, S., van Delft, P., & Balasubramanian, S. (2019) · Nature Chemistry · doi:10.1038/s41557-019-0279-9 · Cited by 83
Metadata
- Authors
- Wu, P., Shui, W., Carlson, B. L., Hu, N., Rabuka, D., Lee, J., & Bertozzi, C. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2009-03-03
- Volume
- 106
- Issue
- 9
- Pages
- 3000-3005
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1979108979
- Cited by
- 237
- References
- 4
- FWCI
- 8.62
- Type
- journal article
- Language
- en
- Open access
- green
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- Wu, P., Shui, W., Carlson, B. L., Hu, N., Rabuka, D., Lee, J., & Bertozzi, C. R. (2009). Site-specific chemical modification of recombinant proteins produced in mammalian cells by using the genetically encoded aldehyde tag. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0807820106
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