The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry
Open accessAbstract
Although the transcriptome, proteome, and interactome of several eukaryotic model organisms have been described in detail, lipidomes remain relatively uncharacterized. Using Saccharomyces cerevisiae as an example, we demonstrate that automated shotgun lipidomics analysis enabled lipidome-wide absolute quantification of individual molecular lipid species by streamlined processing of a single sample of only 2 million yeast cells. By comparative lipidomics, we achieved the absolute quantification of 250 molecular lipid species covering 21 major lipid classes. This analysis provided ≈95% coverage of the yeast lipidome achieved with 125-fold improvement in sensitivity compared with previous approaches. Comparative lipidomics demonstrated that growth temperature and defects in lipid biosynthesis induce ripple effects throughout the molecular composition of the yeast lipidome. This work serves as a resource for molecular characterization of eukaryotic lipidomes, and establishes shotgun lipidomics as a powerful platform for complementing biochemical studies and other systems-level approaches.
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Cites
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Molecular anatomy of a trafficking organelle
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Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast
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Cited by
Modulation of Myelopoiesis Progenitors Is an Integral Component of Trained Immunity
Mitroulis, I., Ruppova, K., Wang, B., Chen, L.-S., Grzybek, M., Grinenko, T., Eugster, A., Troullinaki, M., Palladini, A., Kourtzelis, I., et al. (2018) · Cell · doi:10.1016/j.cell.2017.11.034 · Cited by 1,100
Self-citationAmphotericin primarily kills yeast by simply binding ergosterol
Gray, K. C., Palacios, D. S., Dailey, I., Endo, M. M., Uno, B. E., Wilcock, B. C., & Burke, M. D. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1117280109 · Cited by 608
Liu, L., MacKenzie, K. R., Putluri, N., Maletić-Savatić, M., & Bellen, H. J. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.08.024 · Cited by 556
Amphotericin forms an extramembranous and fungicidal sterol sponge
Anderson, T. M., Clay, M. C., Cioffi, A. G., Diaz, K. A., Hisao, G. S., Tuttle, M. D., Nieuwkoop, A. J., Comellas, G., Maryum, N., Wang, S., et al. (2014) · Nature Chemical Biology · doi:10.1038/nchembio.1496 · Cited by 528
Homeoviscous Adaptation and the Regulation of Membrane Lipids
Ernst, R., Ejsing, C. S., & Antonny, B. (2016) · Journal of Molecular Biology · doi:10.1016/j.jmb.2016.08.013 · Cited by 506
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Metadata
- Authors
- Ejsing, C. S., Sampaio, J. L., Surendranath, V., Duchoslav, E., Ekroos, K., Klemm, R. W., Simons, K., & Shevchenko, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2009-02-17
- Volume
- 106
- Issue
- 7
- Pages
- 2136-2141
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1984954439
- Cited by
- 1,066
- References
- 5
- FWCI
- 30.38
- Type
- journal article
- Language
- en
- Open access
- green
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