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Programmed assembly of 3-dimensional microtissues with defined cellular connectivity
Open accessAbstract
Multicellular organs comprise differentiated cell types with discrete yet interdependent functions. The cells' spatial arrangements and interconnectivities, both critical elements of higher-order function, derive from complex developmental programs in vivo and are often difficult or impossible to emulate in vitro. Here, we report the bottom-up synthesis of microtissues composed of multiple cell types with programmed connectivity. We functionalized cells with short oligonucleotides to impart specific adhesive properties. Hybridization of complementary DNA sequences enabled the assembly of multicellular structures with defined cell–cell contacts. We demonstrated that the kinetic parameters of the assembly process depend on DNA sequence complexity, density, and total cell concentration. Thus, cell assembly can be highly controlled, enabling the design of microtissues with defined cell composition and stoichiometry. We used this strategy to construct a paracrine signaling network in isolated 3-dimensional microtissues.
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Mantovani, A., Allavena, P., Sica, A., & Balkwill, F. (2008) · Nature · doi:10.1038/nature07205 · Cited by 11,399
Lutolf, M. P., & Hubbell, J. A. (2005) · Nature Biotechnology · doi:10.1038/nbt1055 · Cited by 4,551
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Harada, A., Kobayashi, R., Takashima, Y., Hashidzume, A., & Yamaguchi, H. (2011) · Nature Chemistry · doi:10.1038/nchem.893 · Cited by 801
Three-dimensional organotypic culture: experimental models of mammalian biology and disease
Shamir, E. R., & Ewald, A. J. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3873 · Cited by 719
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Chen, Y.-J., Groves, B., Muscat, R. A., & Seelig, G. (2015) · Nature Nanotechnology · doi:10.1038/nnano.2015.195 · Cited by 594
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Wu, M.-H., Huang, S.-B., & Lee, G.-B. (2010) · Lab on a Chip · doi:10.1039/b921695b · Cited by 432
Metadata
- Authors
- Gartner, Z. J., & Bertozzi, C. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2009-03-24
- Volume
- 106
- Issue
- 12
- Pages
- 4606-4610
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2167192225
- Cited by
- 315
- References
- 3
- FWCI
- 6.96
- Type
- journal article
- Language
- en
- Open access
- green
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- Gartner, Z. J., & Bertozzi, C. R. (2009). Programmed assembly of 3-dimensional microtissues with defined cellular connectivity. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0900717106
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
