The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
PDGF signaling is required for epicardial function and blood vessel formation in regenerating zebrafish hearts
Open accessAbstract
A zebrafish heart can fully regenerate after amputation of up to 20% of its ventricle. During this process, newly formed coronary blood vessels revascularize the regenerating tissue. The formation of coronary blood vessels during zebrafish heart regeneration likely recapitulates embryonic coronary vessel development, which involves the activation and proliferation of the epicardium, followed by an epithelial-to-mesenchymal transition. The molecular and cellular mechanisms underlying these processes are not well understood. We examined the role of PDGF signaling in explant-derived primary cultured epicardial cells in vitro and in regenerating zebrafish hearts in vivo. We observed that mural and mesenchymal cell markers, including pdgfr β, are up-regulated in the regenerating hearts. Using a primary culture of epicardial cells derived from heart explants, we found that PDGF signaling is essential for epicardial cell proliferation. PDGF also induces stress fibers and loss of cell-cell contacts of epicardial cells in explant culture. This effect is mediated by Rho-associated protein kinase. Inhibition of PDGF signaling in vivo impairs epicardial cell proliferation, expression of mesenchymal and mural cell markers, and coronary blood vessel formation. Our data suggest that PDGF signaling plays important roles in epicardial function and coronary vessel formation during heart regeneration in zebrafish.
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Cites
Heart Disease and Stroke Statistics—2010 Update: A Report From the American Heart Association
WRITING GROUP MEMBERS, Lloyd-Jones, D., Adams, R. J., Brown, T. M., Carnethon, M., Dai, S., De Simone, G., Ferguson, T. B., Ford, E., Furie, K., et al. (2010) · Circulation · doi:10.1161/circulationaha.109.192667 · Cited by 5,700
Endothelial/Pericyte Interactions
Armulik, A., Abramsson, A., & Betsholtz, C. (2005) · Circulation Research · doi:10.1161/01.res.0000182903.16652.d7 · Cited by 2,048
Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart
Zhou, B., Ma, Q., Rajagopal, S., Wu, S. M., Domian, I., Rivera-Feliciano, J., Jiang, D., von Gise, A., Ikeda, S., Chien, K. R., et al. (2008) · Nature · doi:10.1038/nature07060 · Cited by 1,019
Cited by
The zebrafish as a model for complex tissue regeneration
Gemberling, M., Bailey, T. J., Hyde, D. R., & Poss, K. D. (2013) · Trends in Genetics · doi:10.1016/j.tig.2013.07.003 · Cited by 566
Kikuchi, K., Holdway, J. E., Major, R. J., Blum, N., Dahn, R. D., Begemann, G., & Poss, K. D. (2011) · Developmental Cell · doi:10.1016/j.devcel.2011.01.010 · Cited by 461
Endocardial and Epicardial Epithelial to Mesenchymal Transitions in Heart Development and Disease
von Gise, A., & Pu, W. T. (2012) · Circulation Research · doi:10.1161/circresaha.111.259960 · Cited by 419
Wang, J., Panáková, D., Kikuchi, K., Holdway, J. E., Gemberling, M., Burris, J. S., Singh, S. P., Dickson, A. L., Lin, Y.-F., Sabeh, M. K., et al. (2011) · Development · doi:10.1242/dev.068601 · Cited by 377
Smith, C. L., Baek, S. T., Sung, C. Y., & Tallquist, M. D. (2011) · Circulation Research · doi:10.1161/circresaha.110.235531 · Cited by 339
Metadata
- Authors
- Kim, J., Wu, Q., Zhang, Y., Wiens, K. M., Huang, Y., Rubin, N., Shimada, H., Handin, R. I., Chao, M. Y., Tuan, T.-L., Starnes, V. A., & Lien, C.-L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2010-09-21
- Volume
- 107
- Issue
- 40
- Pages
- 17206-17210
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2072375631
- Cited by
- 197
- References
- 3
- FWCI
- 5.20
- Type
- journal article
- Language
- en
- Open access
- green
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- Kim, J., Wu, Q., Zhang, Y., Wiens, K. M., Huang, Y., Rubin, N., Shimada, H., Handin, R. I., Chao, M. Y., Tuan, T.-L., Starnes, V. A., & Lien, C.-L. (2010). PDGF signaling is required for epicardial function and blood vessel formation in regenerating zebrafish hearts. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0915016107
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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