The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Recapitulation of endochondral bone formation using human adult mesenchymal stem cells as a paradigm for developmental engineering
Open accessAbstract
Mesenchymal stem/stromal cells (MSC) are typically used to generate bone tissue by a process resembling intramembranous ossification, i.e., by direct osteoblastic differentiation. However, most bones develop by endochondral ossification, i.e., via remodeling of hypertrophic cartilaginous templates. To date, endochondral bone formation has not been reproduced using human, clinically compliant cell sources. Here, we aimed at engineering tissues from bone marrow-derived, adult human MSC with an intrinsic capacity to undergo endochondral ossification. By analogy to embryonic limb development, we hypothesized that successful execution of the endochondral program depends on the initial formation of hypertrophic cartilaginous templates. Human MSC, subcutaneously implanted into nude mice at various stages of chondrogenic differentiation, formed bone trabeculae only when they had developed in vitro hypertrophic tissue structures. Advanced maturation in vitro resulted in accelerated formation of larger bony tissues. The underlying morphogenetic process was structurally and molecularly similar to the temporal and spatial progression of limb bone development in embryos. In particular, Indian hedgehog signaling was activated at early stages and required for the in vitro formation of hypertrophic cartilage. Subsequent development of a bony collar in vivo was followed by vascularization, osteoclastic resorption of the cartilage template, and appearance of hematopoietic foci. This study reveals the capacity of human MSC to generate bone tissue via an endochondral program and provides a valid model to study mechanisms governing bone development. Most importantly, this process could generate advanced grafts for bone regeneration by invoking a “developmental engineering” paradigm.
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Cites
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Cited by
Zhang, X., Chen, X., Hong, H., Hu, R., Liu, J., & Liu, C. (2022) · Bioactive Materials · doi:10.1016/j.bioactmat.2021.09.014 · Cited by 680
Biomaterials for articular cartilage tissue engineering: Learning from biology
Armiento, A. R., Stoddart, M. J., Alini, M., & Eglin, D. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.11.021 · Cited by 579
Engineering of a functional bone organ through endochondral ossification
Scotti, C., Piccinini, E., Takizawa, H., Todorov, A., Bourgine, P., Papadimitropoulos, A., Barbero, A., Manz, M. G., & Martin, I. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1220108110 · Cited by 321
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Xu, T., Miszuk, J. M., Zhao, Y., Sun, H., & Fong, H. (2015) · Advanced Healthcare Materials · doi:10.1002/adhm.201500345 · Cited by 282
Metadata
- Authors
- Scotti, C., Tonnarelli, B., Papadimitropoulos, A., Scherberich, A., Schaeren, S., Schauerte, A., Lopez-Rios, J., Zeller, R., Barbero, A., & Martin, I.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2010-04-06
- Volume
- 107
- Issue
- 16
- Pages
- 7251-7256
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2096467350
- Cited by
- 477
- References
- 39
- FWCI
- 15.84
- Type
- journal article
- Language
- en
- Open access
- green
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