The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Positive regulation by small RNAs and the role of Hfq
Open accessAbstract
Bacterial small noncoding RNAs carry out both positive and negative regulation of gene expression by pairing with mRNAs; in Escherichia coli , this regulation often requires the RNA chaperone Hfq. Three small regulatory RNAs (sRNAs), DsrA, RprA, and ArcZ, positively regulate translation of the sigma factor RpoS, each pairing with the 5′ leader to open up an inhibitory hairpin. In vitro, rpoS interaction with sRNAs depends upon an (AAN) 4 Hfq-binding site upstream of the pairing region. Here we show that both Hfq and this Hfq binding site are required for RprA or ArcZ to act in vivo and to form a stable complex with rpoS mRNA in vitro; both were partially dispensable for DsrA at 37 °C. ArcZ sRNA is processed from 121 nt to a stable 56 nt species that contains the pairing region; only the 56 nt ArcZ makes a strong Hfq-dependent complex with rpoS . For each of these sRNAs, the stability of the sRNA•mRNA complexes, rather than their rate of formation, best predicted in vivo activity. These studies demonstrate that binding of Hfq to the rpoS mRNA is critical for sRNA regulation under normal conditions, but if the stability of the sRNA•mRNA complex is sufficiently high, the requirement for Hfq can be bypassed.
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Cites
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The OxyS regulatory RNA represses rpoS translation and binds the Hfq (HF-I) protein
Zhang, A. (1998) · The EMBO Journal · doi:10.1093/emboj/17.20.6061 · Cited by 784
Novel small RNA-encoding genes in the intergenic regions of Escherichia coli
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The Small RNA Regulators of Escherichia coli : Roles and Mechanisms
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Self-citationSittka, A., Lucchini, S., Papenfort, K., Sharma, C. M., Rolle, K., Binnewies, T. T., Hinton, J. C. D., & Vogel, J. (2008) · PLoS Genetics · doi:10.1371/journal.pgen.1000163 · Cited by 565
Global analysis of small RNA and mRNA targets of Hfq
Zhang, A., Wassarman, K. M., Rosenow, C., Tjaden, B. C., Storz, G., & Gottesman, S. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03734.x · Cited by 544
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Cited by
Regulatory RNA in Bacterial Pathogens
Papenfort, K., & Vogel, J. (2010) · Cell Host & Microbe · doi:10.1016/j.chom.2010.06.008 · Cited by 324
Integrating anaerobic/aerobic sensing and the general stress response through the ArcZ small RNA
Mandin, P., & Gottesman, S. (2010) · The EMBO Journal · doi:10.1038/emboj.2010.179 · Cited by 288
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Metadata
- Authors
- Soper, T., Mandin, P., Majdalani, N., Gottesman, S., & Woodson, S. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2010-05-08
- Volume
- 107
- Issue
- 21
- Pages
- 9602-9607
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2057587371
- Cited by
- 273
- References
- 38
- FWCI
- 16.93
- Type
- journal article
- Language
- en
- Open access
- green
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