Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice
Open accessAbstract
Transposable elements have been routinely used for genetic manipulation in lower organisms, including generating transgenic animals and insertional mutagenesis. In contrast, the usage of transposons in mice and other vertebrate systems is still limited due to the lack of an efficient transposition system. We have tested the ability of piggyBac (PB), a DNA transposon from the cabbage looper moth Trichoplusia ni, to transpose in mammalian systems. We show that PB elements carrying multiple genes can efficiently transpose in human and mouse cell lines and also in mice. PB permits the expression of the marker genes it carried. During germline transposition, PB could excise precisely from original insertion sites and transpose into the mouse genome at diverse locations, preferably transcription units. These data provide a first and critical step toward a highly efficient transposon system for a variety of genetic manipulations including transgenesis and insertional mutagenesis in mice and other vertebrates.
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The PiggyBac transposon system uses an efficient transposase purified from the cabbage looper to integrate the gene of interest into the host genome.
“Also, the PiggyBac™ (System Bioscience inc.) transposon system uses an efficient transposase purified from the cabbage looper (Trichoplusia ni) to easily integrate the gene of interest into the host genome (Ding et al., 2005).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Ding et al., 2005
The supplied passage and the additional indexed passages have no section or page labels and contain unrelated, repetitive Arabic text. Searches for the cabbage looper, purified transposase, and genomic integration found no passage in our copy that addresses the claim. (LLM, conf=0.78)
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- Authors
- Ding, S., Wu, X., Li, G., Han, M., Zhuang, Y., & Xu, T.
- Affiliations
- Fudan University (CN)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Wu, Xiaohui
- Xu, Tian
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2005-08
- Volume
- 122
- Issue
- 3
- Pages
- 473-483
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2123784922
- Cited by
- 1,009
- References
- 1
- Citations by year
- 2026: 45 · 2025: 65 · 2024: 40 · 2023: 52 · 2022: 44 · 2021: 32 · 2020: 55 · 2019: 42 · 2018: 47 · 2017: 46 · 2016: 50 · 2015: 56 · 2014: 64 · 2013: 63 · 2012: 52
- FWCI
- 12.09
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Keywords
- piggyBac transposon, DNA transposons, transposition efficiency, germline transposition, transgenesis, insertional mutagenesis, transposon excision, Trichoplusia ni, mouse genome, mammalian cell lines, marker gene expression
- MeSH terms
- Animals, Cell Line, DNA Transposable Elements, Gene Expression Regulation, Genetic Vectors, Humans, Mice, Transgenic, Transformation, Genetic, Mutagenesis, Mice
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