The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Comprehensive microRNA expression profiling of the hematopoietic hierarchy
Open accessAbstract
The hematopoietic system produces a large number of highly specialized cell types that are derived through a hierarchical differentiation process from a common stem cell population. miRNAs are critical players in orchestrating this differentiation. Here, we report the development and application of a high-throughput microfluidic real-time quantitative PCR (RT-qPCR) approach for generating global miRNA profiles for 27 phenotypically distinct cell populations isolated from normal adult mouse hematopoietic tissues. A total of 80,000 RT-qPCR assays were used to map the landscape of miRNA expression across the hematopoietic hierarchy, including rare progenitor and stem cell populations. We show that miRNA profiles allow for the direct inference of cell lineage relations and functional similarity. Our analysis reveals a close relatedness of the miRNA expression patterns in multipotent progenitors and stem cells, followed by a major reprogramming upon restriction of differentiation potential to a single lineage. The analysis of miRNA expression in single hematopoietic cells further demonstrates that miRNA expression is very tightly regulated within highly purified populations, underscoring the potential of single-cell miRNA profiling for assessing compartment heterogeneity.
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Cites
A clonogenic common myeloid progenitor that gives rise to all myeloid lineages
Akashi, K., Traver, D., Miyamoto, T., & Weissman, I. L. (2000) · Nature · doi:10.1038/35004599 · Cited by 2,617
Cited by
MicroRNA profiling: approaches and considerations
Pritchard, C. C., Cheng, H. H., & Tewari, M. (2012) · Nature Reviews Genetics · doi:10.1038/nrg3198 · Cited by 1,765
High-throughput microfluidic single-cell RT-qPCR
White, A. K., VanInsberghe, M., Petriv, O. I., Hamidi, M., Sikorski, D., Marra, M. A., Piret, J., Aparicio, S., & Hansen, C. L. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1019446108 · Cited by 440
Self-citationAdult-specific functions of animal microRNAs
Sun, K., & Lai, E. C. (2013) · Nature Reviews Genetics · doi:10.1038/nrg3471 · Cited by 334
Micro Total Analysis Systems for Cell Biology and Biochemical Assays
Kovarik, M. L., Gach, P. C., Ornoff, D. M., Wang, Y., Balowski, J., Farrag, L., & Allbritton, N. L. (2012) · Analytical Chemistry · doi:10.1021/ac202611x · Cited by 270
Tian, Q., Price, N. D., & Hood, L. (2012) · Journal of Internal Medicine · doi:10.1111/j.1365-2796.2011.02498.x · Cited by 224
Metadata
- Authors
- Petriv, O. I., Kuchenbauer, F., Delaney, A. D., Lecault, V., White, A., Kent, D., Marmolejo, L., Heuser, M., Berg, T., Copley, M., Ruschmann, J., Sekulovic, S., Benz, C., Kuroda, E., Ho, V., Antignano, F., Halim, T., Giambra, V., Krystal, G., Takei, C. J. F., Weng, A. P., Piret, J., Eaves, C., Marra, M. A., Humphries, R. K., & Hansen, C. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2010-08-11
- Volume
- 107
- Issue
- 35
- Pages
- 15443-15448
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2017971576
- Cited by
- 170
- References
- 1
- FWCI
- 5.51
- Type
- journal article
- Language
- en
- Open access
- green
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- Petriv, O. I., Kuchenbauer, F., Delaney, A. D., Lecault, V., White, A., Kent, D., Marmolejo, L., Heuser, M., Berg, T., Copley, M., Ruschmann, J., Sekulovic, S., Benz, C., Kuroda, E., Ho, V., Antignano, F., Halim, T., Giambra, V., Krystal, G., . . . Hansen, C. L. (2010). Comprehensive microRNA expression profiling of the hematopoietic hierarchy. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1009320107
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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