The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation
Open accessAbstract
The use of plant biomass for biofuel production will require efficient utilization of the sugars in lignocellulose, primarily glucose and xylose. However, strains of Saccharomyces cerevisiae presently used in bioethanol production ferment glucose but not xylose. Yeasts engineered to ferment xylose do so slowly, and cannot utilize xylose until glucose is completely consumed. To overcome these bottlenecks, we engineered yeasts to coferment mixtures of xylose and cellobiose. In these yeast strains, hydrolysis of cellobiose takes place inside yeast cells through the action of an intracellular β-glucosidase following import by a high-affinity cellodextrin transporter. Intracellular hydrolysis of cellobiose minimizes glucose repression of xylose fermentation allowing coconsumption of cellobiose and xylose. The resulting yeast strains, cofermented cellobiose and xylose simultaneously and exhibited improved ethanol yield when compared to fermentation with either cellobiose or xylose as sole carbon sources. We also observed improved yields and productivities from cofermentation experiments performed with simulated cellulosic hydrolyzates, suggesting this is a promising cofermentation strategy for cellulosic biofuel production. The successful integration of cellobiose and xylose fermentation pathways in yeast is a critical step towards enabling economic biofuel production.
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Cites
The Path Forward for Biofuels and Biomaterials
Ragauskas, A. J., Williams, C. K., Davison, B. H., Britovsek, G., Cairney, J., Eckert, C. A., Frederick, W. J., Hallett, J. P., Leak, D. J., Liotta, C. L., et al. (2006) · Science · doi:10.1126/science.1114736 · Cited by 5,741
Towards industrial pentose-fermenting yeast strains
Hahn-Hägerdal, B., Karhumaa, K., Fonseca, C., Spencer-Martins, I., & Gorwa-Grauslund, M. F. (2007) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-006-0827-2 · Cited by 783
Challenges in Engineering Microbes for Biofuels Production
Stephanopoulos, G. (2007) · Science · doi:10.1126/science.1139612 · Cited by 731
Carroll, A., & Somerville, C. (2009) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.043008.092125 · Cited by 676
What is (and is not) vital to advancing cellulosic ethanol
Wyman, C. E. (2007) · Trends in Biotechnology · doi:10.1016/j.tibtech.2007.02.009 · Cited by 642
Genetically Engineered Saccharomyces Yeast Capable of Effective Cofermentation of Glucose and Xylose
Ho, N. W. Y., Chen, Z., & Brainard, A. P. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.5.1852-1859.1998 · Cited by 540
Xylose fermentation by Saccharomyces cerevisiae
K�tter, P., & Ciriacy, M. (1993) · Applied Microbiology and Biotechnology · doi:10.1007/bf00167144 · Cited by 509
Metabolic engineering for improved fermentation of pentoses by yeasts
Jeffries, T. W., & Jin, Y.-S. (2004) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-003-1450-0 · Cited by 499
Self-citationGenome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis
Jeffries, T. W., Grigoriev, I. V., Grimwood, J., Laplaza, J. M., Aerts, A., Salamov, A., Schmutz, J., Lindquist, E., Dehal, P., Shapiro, H., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1290 · Cited by 494
Self-citationEliasson, A., Christensson, C., Wahlbom, C. F., & Hahn-Hägerdal, B. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.8.3381-3386.2000 · Cited by 392
Cited by
Review of Second-Generation Bioethanol Production from Residual Biomass
Lodz University of Technology, F. O. B. A. F. S., Robak, K., Balcerek, M., & Lodz University of Technology, F. O. B. A. F. S. (2018) · Food Technology and Biotechnology · doi:10.17113/ftb.56.02.18.5428 · Cited by 649
Current challenges in commercially producing biofuels from lignocellulosic biomass
Balan, V. (2014) · ISRN Biotechnology · doi:10.1155/2014/463074 · Cited by 551
Hong, K.-K., & Nielsen, J. (2012) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-012-0945-1 · Cited by 451
Bio‐based production of C2–C6 platform chemicals
Jang, Y., Kim, B., Shin, J. H., Choi, Y. J., Choi, S., Song, C. W., Lee, J., Park, H. G., & Lee, S. Y. (2012) · Biotechnology and Bioengineering · doi:10.1002/bit.24599 · Cited by 386
Selection of chromosomal DNA libraries using a multiplex CRISPR system
Ryan, O. W., Skerker, J. M., Maurer, M. J., Li, X., Tsai, J. C., Poddar, S., Lee, M. E., DeLoache, W., Dueber, J. E., Arkin, A. P., et al. (2014) · eLife · doi:10.7554/elife.03703 · Cited by 373
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Metadata
- Authors
- Ha, S.-J., Galazka, J. M., Rin Kim, S., Choi, J.-H., Yang, X., Seo, J.-H., Louise Glass, N., Cate, J. H. D., & Jin, Y.-S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2010-12-27
- Volume
- 108
- Issue
- 2
- Pages
- 504-509
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2147913192
- Cited by
- 466
- References
- 27
- FWCI
- 20.02
- Type
- journal article
- Language
- en
- Open access
- green
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- Ha, S.-J., Galazka, J. M., Rin Kim, S., Choi, J.-H., Yang, X., Seo, J.-H., Louise Glass, N., Cate, J. H. D., & Jin, Y.-S. (2011). Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1010456108
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