Cited by 277HIV Research and TreatmentHIV/AIDS drug development and treatmentT-cell and Retrovirus Studies

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Complementary assays reveal a relationship between HIV-1 uncoating and reverse transcription

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Hulme, Amy E., Perez, Omar, Hope, Thomas J.·2011·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.1014522108PMC PMC3116424

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Abstract

During the early stages of HIV-1 replication the conical capsid composed of p24 CA protein dissociates from the rest of the cytoplasmic viral complex by a process called uncoating. Although proper uncoating is known to be required for HIV-1 infection, many questions remain about the timing and factors involved in the process. Here we have used two complementary assays to study the process of uncoating in HIV-1–infected cells, specifically looking at the timing of uncoating and its relationship to reverse transcription. We developed a fluorescent microscopy-based uncoating assay that detects the association of p24 CA with HIV-1 viral complexes in cells. We also used an owl monkey kidney (OMK) cell assay that is based on timed TRIM-CypA–mediated restriction of HIV-1 replication. Results from both assays indicate that uncoating is initiated within 1 h of viral fusion. In addition, treatment with the reverse transcriptase inhibitor nevirapine delayed uncoating in both assays. Analysis of reverse transcription products in OMK cells revealed that the generation of early reverse transcription products coincides with the timing of uncoating in these assays. Collectively, these results suggest that some aspect of reverse transcription has the ability to influence the kinetics of uncoating.

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Authors: Hulme, A. E., Perez, O., & Hope, T. J.
Affiliations: Northwestern University (US)
University of Illinois Chicago (US)
ORCID: Hulme, Amy E.
Perez, Omar
Hope, Thomas J.
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2011-05-31
Volume: 108
Issue: 24
Pages: 9975-9980
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.1014522108
PMC: PMC3116424
OpenAlex: W2120792521
Cited by: 276
References: 29
Citations by year: 2026: 6 · 2025: 8 · 2024: 14 · 2023: 9 · 2022: 18 · 2021: 24 · 2020: 24 · 2019: 20 · 2018: 23 · 2017: 15 · 2016: 19 · 2015: 15 · 2014: 24 · 2013: 27 · 2012: 22
FWCI: 10.25
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
BearWorks (Missouri State University) (submittedVersion)
Funders: National Institute of General Medical Sciences (P50GM082545)
National Institute of Allergy and Infectious Diseases (R01-AI47770, R01AI047770)
Topics: Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
Health Sciences › Medicine › Infectious Diseases › HIV/AIDS drug development and treatment
Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
Keywords: reverse transcription, viral capsid, viral fusion, nevirapine
MeSH terms: Animals, Cell Line, HeLa Cells, Humans, Luminescent Proteins, Microscopy, Fluorescence, Recombinant Fusion Proteins, Time Factors, Virion, Virus Replication, HIV-1, Cyclosporine, HIV Core Protein p24, Macrolides, Anti-HIV Agents, Nevirapine, Reverse Transcription, Green Fluorescent Proteins, Human Immunodeficiency Virus Proteins, vpr Gene Products, Human Immunodeficiency Virus, HEK293 Cells

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Complementary assays reveal a relationship between HIV-1 uncoating and reverse transcription

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