The DOI matches its Crossref record; 44 of the 45 references it cites were found in a registry or an index.
Calcium-dependent dynamics of cadherin interactions at cell–cell junctions
Open accessAbstract
Cadherins play a key role in the dynamics of cell–cell contact formation and remodeling of junctions and tissues. Cadherin–cadherin interactions are gated by extracellular Ca 2+ , which serves to rigidify the cadherin extracellular domains and promote trans junctional interactions. Here we describe the direct visualization and quantification of spatiotemporal dynamics of N -cadherin interactions across intercellular junctions in living cells using a genetically encodable FRET reporter system. Direct measurements of transjunctional cadherin interactions revealed a sudden, but partial, loss of homophilic interactions (τ = 1.17 ± 0.06 s −1 ) upon chelation of extracellular Ca 2+ . A cadherin mutant with reduced adhesive activity (W2A) exhibited a faster, more substantial loss of homophilic interactions (τ = 0.86 ± 0.02 s −1 ), suggesting two types of native cadherin interactions—one that is rapidly modulated by changes in extracellular Ca 2+ and another with relatively stable adhesive activity that is Ca 2+ independent. The Ca 2+ -sensitive dynamics of cadherin interactions were transmitted to the cell interior where β-catenin translocated to N -cadherin at the junction in both cells. These data indicate that cadherins can rapidly convey dynamic information about the extracellular environment to both cells that comprise a junction.
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“Cadherins regulate calcium-dependent cell–cell adherent junctions, where the chelation of calcium abolishes adhesive activity and allows proteolytic degradation of cadherins (Nagar and Overduint, 1996; Kim et al., 2011).”
From APOE3 Christchurch modulates β-catenin/Wnt signaling in iPS cell-derived cerebral organoids from Alzheimer’s cases (2024) · cites this paper as Kim et al., 2011
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Structural basis of calcium-induced E-cadherin rigidification and dimerization
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C-Cadherin Ectodomain Structure and Implications for Cell Adhesion Mechanisms
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Research works printed but not linked: 1
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Metadata
- Authors
- Kim, S. A., Tai, C.-Y., Mok, L.-P., Mosser, E. A., & Schuman, E. M.
- Affiliations
- California Institute of Technology (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2011-05-25
- Volume
- 108
- Issue
- 24
- Pages
- 9857-9862
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC3116393
- OpenAlex
- W2071583976
- Cited by
- 170
- References
- 45
- Citations by year
- 2026: 13 · 2025: 9 · 2024: 22 · 2023: 17 · 2022: 17 · 2021: 10 · 2020: 15 · 2019: 13 · 2018: 7 · 2017: 8 · 2016: 10 · 2015: 10 · 2014: 9 · 2013: 7 · 2012: 3
- FWCI
- 4.52
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- Topics
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connexins and lens biology
- Keywords
- cadherins, N-cadherin, homophilic interactions, calcium signaling, cell-cell contact, FRET reporter, β-catenin nuclear translocation, cell adhesion, junction remodeling
- MeSH terms
- Animals, Calcium, Chlorocebus aethiops, Humans, Intercellular Junctions, Kinetics, L Cells, Protein Binding, Recombinant Fusion Proteins, Transfection, Cadherins, Microscopy, Confocal, COS Cells, Fluorescence Resonance Energy Transfer, Green Fluorescent Proteins, beta Catenin, Mice, HEK293 Cells
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